Kallikrein 2 Polyclonal antibody

Kallikrein 2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 10812-1-AP

Synonyms

Glandular kallikrein 1, hGK 1, hK2, Kallikrein 2, kallikrein related peptidase 2, KLK2, KLK2A2, Tissue kallikrein 2

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Validation Data Gallery

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  WB analysis of mouse pancreas using 10812-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 10812-1-AP (Kallikrein 2 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 10812-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10812-1-AP (Kallikrein 2 antibody) at dilution of 1:100 (under 10x lens).

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Tested Applications

• Positive WB detected in: mouse pancreas tissue
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

10812-1-AP targets Kallikrein 2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Kallikrein 2 fusion protein Ag1077
• Full Name: kallikrein-related peptidase 2
• Calculated Molecular Weight: 28 kDa
• Observed Molecular Weight: 29 kda
• GenBank Accession Number: BC005196
• Gene Symbol: KLK2
• Gene ID (NCBI): 3817
• RRID: AB_2134232
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KLK2(Kallikrein-2) is also named as hGK-1 and belongs to the Kallikrein subfamily. It plays a role in prostate cancer susceptibility and this role may be realized at least in part by the induction of increases in its production. It also may mediate tumor growth and invasion by participating in proteolytic cascades. This protein has 4 isoforms produced by alternative splicing with the molecular weight of 29 kDa, 25 kDa, 18 kDa and 17 kDa.

Publications

Species	Application	Title
human	IHC,WB	J Photochem Photobiol B; Inhibition of hypoxia-inducible factor 1 by acriflavine renders glioblastoma sensitive for photodynamic therapy; Authors - Shuai Ma
human	WB	Cancer Med; Par14 interacts with the androgen receptor, augmenting both its transcriptional activity and prostate cancer proliferation; Authors - Miki Naito