KLHL14-Specific Polyclonal antibody

• Featured Product
• KD/KO Validated

KLHL14-Specific Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 14849-1-AP

Synonyms

Protein interactor of Torsin-1A, Printor, KLHL14, KIAA1384, Kelch-like protein 14

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Validation Data Gallery

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  WB analysis of HeLa using 14849-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 14849-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 14849-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 14849-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14849-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 14849-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 14849-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 14849-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 14849-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 14849-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 14849-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 14849-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human cervical cancer using 14849-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14849-1-AP (KLHL14-Specific Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 14849-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14849-1-AP (KLHL14-Specific Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 14849-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14849-1-AP (KLHL14-Specific Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human cervical cancer using 14849-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14849-1-AP (KLHL14-Specific Antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HepG2 using 14849-1-AP

  Immunofluorescent analysis of HepG2 cells, using KLHL14 antibody 14849-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse heart tissue, mouse testis tissue, mouse lung tissue, mouse liver tissue, SH-SY5Y cells, mouse brain tissue, mouse ovary tissue, HepG2 cells, rat brain tissue
• Positive IHC detected in: human heart tissue, human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

14849-1-AP targets KLHL14-Specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: kelch-like 14 (Drosophila)
• Calculated Molecular Weight: 70 kDa
• Observed Molecular Weight: 39-45 kDa
• GenBank Accession Number: NM_020805
• Gene Symbol: KLHL14
• Gene ID (NCBI): 57565
• RRID: AB_2131171
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KLHL14, a member of the Kelch-like gene family, has six Kelch domains and is localized to the endoplasmic reticulum. KLHL14, a novel tumor suppressor, is a subunit of a ubiquitin ligase that associates with the endoplasmic reticulum-associated protein degradation (ERAD) machinery, regulating of B cell receptor-dependent NF-κB signaling (PMID: 32127472). KLHL14 has two isoforms of 70kDa and 43kDa.

Publications

Species	Application	Title
human	WB	Int J Clin Exp Pathol; Kelch-like protein 14 promotes proliferation and migration of ovarian cancer cells.; Authors - Zheng Chen ; KD Validated