KLF9 Recombinant antibody

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  WB analysis using 83183-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83183-4-RR (KLF9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  FC experiment of A549 using 83183-4-RR

  1x10^6 A549 cells were intracellularly stained with 0.25 ug KLF9 Recombinant antibody (83183-4-RR, Clone:230436H2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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  Affinity and Kinetic Characterization of 83183-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83183-4-RR against Human KLF9 were performed. The affinity constant is 0.767 nM.

KLF9 {ptg:Brand} Recombinant Antibody for WB, FC (Intra), ELISA

Cat No. 83183-4-RR

Clone No.230436H2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

GC-box-binding protein 1, BTE-binding protein 1, BTEB1, BTEB, Basic transcription element-binding protein 1

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Tested Applications

• Positive WB detected in: HeLa cells, K-562 cells, HepG2 cells, A549 cells, U2OS cells
• Positive FC (Intra) detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83183-4-RR targets KLF9 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: KLF9 fusion protein Ag34411
• Full Name: Kruppel-like factor 9
• Calculated Molecular Weight: 27 kDa
• Observed Molecular Weight: 27-35 kDa
• GenBank Accession Number: BC074880
• Gene Symbol: KLF9
• Gene ID (NCBI): 687
• RRID: AB_3670873
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q13886
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Krüppel-like factor 9 (KLF9) was a member of SP/KLF family of DNA-binding transcriptional regulators featured by a highly homologous C-terminal three C2H2-type zinc finger DNA-binding domains. KLF9 has been previously reported to be downregulated in various types of human malignancy and serves as a tumour suppressor at tumour onset and development, affecting cell proliferation, apoptosis, metastasis and tumorigenicity (PMID: 32855660).