KLF2 Polyclonal antibody

• 

  WB analysis using 23384-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23384-1-AP (KLF2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• Featured Product
• KD/KO Validated

KLF2 Polyclonal Antibody for WB, ELISA

Cat No. 23384-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ChIP, ELISA

Synonyms

KLF2, Krueppel like factor 2, Kruppel like factor 2 (lung), LKLF, Lung krueppel like factor

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Tested Applications

• Positive WB detected in: HepG2 cells, bEnd.3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IP: See 1 publications below
• ChIP: See 1 publications below

Product Information

23384-1-AP targets KLF2 in WB, IP, ChIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KLF2 fusion protein Ag20019
• Full Name: Kruppel-like factor 2 (lung)
• Calculated Molecular Weight: 355 aa, 37 kDa
• Observed Molecular Weight: 37-40 kDa, 60 kDa
• GenBank Accession Number: BC071983
• Gene Symbol: KLF2
• Gene ID (NCBI): 10365
• RRID: AB_3085710
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• UNIPROT ID: Q9Y5W3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KLF2 (Kruppel-like Factor 2) belongs to zinc finger transcription factors, and it is implicated in a variety of biochemical processes, including lung development, embryonic erythropoiesis, epithelial integrity, T-cell viability, and adipogenesis (PMID: 17904406). KLF2 was identified as a negative regulator of T cell activation and adipogenesis. KLF2 also acts as a novel regulator of endothelial proinflammatory activation, inhibiting the inflammatory cytokine-mediated induction of adhesion molecule expression and immune cell adhesion (PMID:15718498). KLF2-null mice exhibit abnormal blood vessel formation, resulting in embryonic haemorrhage and death (PMID: 32132543). KLF2 can be detected 60 kDa by post-translationally modified (PMID: 36466816).

Publications

Species	Application	Title
human	WB,IP	J Thromb Haemost; IKKβ regulates endothelial thrombomodulin in a Klf2-dependent manner.; Authors - R Pathak
human	ChIP	J Cardiothorac Surg; Mechanism of KLF2 in young mice with pneumonia induced by Streptococcus pneumoniae; Authors - Xiaoshuang Li
human,mouse	WB	Drug Dev Res; KLF2 Inhibits Ferroptosis and Improves Mitochondrial Dysfunction in Chondrocyte Through SIRT1/GPX4 Signaling to Improve Osteoarthritis; Authors - Jiaqi Shi