KLF15 Polyclonal antibody

• Featured Product
• KD/KO Validated

KLF15 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, ChIP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 13749-1-AP

Synonyms

KKLF, KLF15, Krueppel like factor 15, Kruppel like factor 15

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Validation Data Gallery

• 

  WB analysis using 13749-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13749-1-AP (KLF15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13749-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13749-1-AP (KLF15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: MCF-7 cells, MDA-MB-231 cells, 3T3-L1 cells, C2C12 cells, mouse heart tissue, mouse kidney tissue, mouse liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• ChIP: See 1 publications below

Product Information

13749-1-AP targets KLF15 in WB, ChIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KLF15 fusion protein Ag4690
• Full Name: Kruppel-like factor 15
• Calculated Molecular Weight: 416 aa, 44 kDa
• Observed Molecular Weight: 44 kDa
• GenBank Accession Number: BC036733
• Gene Symbol: KLF15
• Gene ID (NCBI): 28999
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KLF15, a kidney-enriched Krüppel-like zinc finger-containing transcription factors, is a transcriptonal activator that binds the CLCNKA promoter. The nuclear protein KLF15, is expressed primarily in liver, heart, skeletal muscle and kidney tissues but is not detected in lymphoid tissues or bone marrow. It is an important regulator of GLUT4 in both adipose and muscle tissues.Although the calculated molecular mass of KLF15 is 44 kDa, the apparent molecular mass on SDS-PAGE is 65 kDa due to the post-translational modifications (PMID:10982849,PMID:16615998).

Publications

Species	Application	Title
rat	WB,ChIP	Cell Metab; Crosstalk between glucocorticoid receptor and nutritional sensor mTOR in skeletal muscle.; Authors - Noriaki Shimizu
human	WB	Mol Cell Biol; A remodeled Hsp90 molecular chaperone ensemble with the novel co-chaperone Aarsd1 is required for muscle differentiation.; Authors - Pablo C Echeverría
mouse	WB	PLoS One; PMID: 24167585; Authors - Jung Dae Young DY ; KO Validated