KLF10 Polyclonal antibody

KLF10 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29709-1-AP

Synonyms

EGR alpha, EGRA, KLF10, Krueppel like factor 10, Kruppel like factor 10, TIEG, TIEG 1, TIEG1

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Validation Data Gallery

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  WB analysis using 29709-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29709-1-AP (KLF10 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 29709-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29709-1-AP (KLF10 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse pancreas using 29709-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 29709-1-AP (KLF10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse pancreas using 29709-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 29709-1-AP (KLF10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Jurkat cells, K-562 cells, MCF-7 cells
• Positive IHC detected in: mouse pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29709-1-AP targets KLF10 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KLF10 fusion protein Ag31772
• Full Name: Kruppel-like factor 10
• Calculated Molecular Weight: 469 aa, 51 kDa
• Observed Molecular Weight: 52-75 kDa
• GenBank Accession Number: BC011538
• Gene Symbol: KLF10
• Gene ID (NCBI): 7071
• RRID: AB_2918341
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KLF10 (Krueppel-like factor 10), also known as TIEG1, is a transcription factor that plays a role in the regulation of cell growth. KLF10 plays a role in the response to TGF beta (through the TGF beta/Smad signaling pathway) and estrogen. KLF10 has been shown to have a role in skeletal disease (osteopenia/osteoporosis), hypertrophic cardiomyopathy, and breast and prostate cancer. KLF10 can be detected 51-52 kDa, 60-75 kDa (PMID: 21262377, PMID: 30026232).