KL Monoclonal antibody

KL Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, rat and More (1)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

4D1G11

Cat no : 67331-1-Ig

Synonyms

KL, klotho

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Validation Data Gallery

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  WB analysis of mouse kidney using 67331-1-Ig

  Mouse kidney tissue were subjected to SDS PAGE followed by western blot with 67331-1-Ig (KL antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 2 publications below

Product Information

67331-1-Ig targets KL in WB, IF, ELISA applications and shows reactivity with Human, Mouse, rat samples.

• Tested Reactivity: Human, Mouse, rat
• Cited Reactivity: mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: KL fusion protein Ag27838
• Full Name: klotho
• Calculated Molecular Weight: 116 kDa
• Observed Molecular Weight: 125-135 kDa
• GenBank Accession Number: NM_004795
• Gene Symbol: KL
• Gene ID (NCBI): 9365
• RRID: AB_2882590
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
rat	IF	Toxicol Lett; Damage of uremic myocardium by p-cresyl sulfate and the ameliorative effect of Klotho by regulating SIRT6 ubiquitination.; Authors - Cheng Chen
mouse	IF	Apoptosis; Vitamin D inhibits ferroptosis and mitigates the kidney injury of prediabetic mice by activating the Klotho/p53 signaling pathway; Authors - Hao Chen