CD117/c-Kit Polyclonal antibody

CD117/c-Kit Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (4)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

29

Cat no : 18696-1-AP

Synonyms

CD117, KIT, Mast/stem cell growth factor receptor Kit, EC:2.7.10.1, c-Kit

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Validation Data Gallery

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  WB analysis of Mo7e using 18696-1-AP

  Mo7e cells were subjected to SDS PAGE followed by western blot with 18696-1-AP (CD117/c-kit antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of stromal tumor using 18696-1-AP

  Immunohistochemical analysis of paraffin-embedded stromal tumor tissue slide using 18696-1-AP (CD117/c-kit antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of stromal tumor using 18696-1-AP

  Immunohistochemical analysis of paraffin-embedded stromal tumor tissue slide using 18696-1-AP (CD117/c-kit antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human placenta using 18696-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 18696-1-AP (CD117/c-kit antibody) at dilution of 1:400 (under 40x lens).

• 

  IHC staining of human testis using 18696-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18696-1-AP (CD117/c-kit antibody) at dilution of 1:400 (under 40x lens).

• 

  IHC staining of Insulinoma using 18696-1-AP

  Immunohistochemical analysis of paraffin-embedded Insulinoma tissue slide using 18696-1-AP (CD117/c-kit antibody) at dilution of 1:2000 (under 10x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

• 

  IHC staining of Insulinoma using 18696-1-AP

  Immunohistochemical analysis of paraffin-embedded Insulinoma tissue slide using 18696-1-AP (CD117/c-kit antibody) at dilution of 1:2000 (under 40x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

• 

  IF Staining of MCF-7 using 18696-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 18696-1-AP (CD117/c-kit antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: Mo7e cells
• Positive IHC detected in: stromal tumor tissue, human placenta tissue, human testis tissue, Insulinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IHC: See 11 publications below
• IF: See 9 publications below

Product Information

18696-1-AP targets CD117/c-Kit in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat, sheep, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
• Calculated Molecular Weight: 110 kDa
• Observed Molecular Weight: 140-160 kDa
• GenBank Accession Number: BC071593
• Gene Symbol: CD117
• Gene ID (NCBI): 3815
• RRID: AB_2249558
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD117, also known as c-Kit and SCFR, is a transmembrane protein with tyrosine kinase activity encoded by the oncogene c-kit (PMID: 2448137). It is a member of the type III receptor tyrosine kinase family, which also includes CSF-1R, PDGFRβ, PDGFRα, and FLT3 (PMID: 29518044). CD117 is expressed on hematopoietic stem cells and progenitor cells, mast cells, and is also found in a wide range of non-haemopoietic cell types (including melanocytes, germ cells, astrocytes, renal tubules, breast glandular epithelial cells, sweat glands, and interstitial cells of Cajal) (PMID: 10582338; 23073628). CD117 plays an important role in early hemopoiesis. It is also involved in pigmentation, fertility, gut movement, and some aspects of the nervous system (PMID: 23073628).

Publications

Species	Application	Title
human	WB	Mech Ageing Dev; Acceleration of melanocyte senescence by the proinflammatory cytokines IFNγ and TNFα impairs the repigmentation response of vitiligo patients to narrowband ultraviolet B (NBUVB) phototherapy; Authors - Bing-Qi Dong
human	IHC	Front Cell Dev Biol; Single-Cell Sequencing Unveils the Heterogeneity of Nonimmune Cells in Chronic Apical Periodontitis.; Authors - Xinwei Lin
human	IHC	Front Immunol; Immune Infiltrating Cells-Derived Risk Signature Based on Large-scale Analysis Defines Immune Landscape and Predicts Immunotherapy Responses in Glioma Tumor Microenvironment.; Authors - Nan Zhang
human	IF	J Exp Clin Cancer Res; Growth differentiation factor 15 contributes to marrow adipocyte remodeling in response to the growth of leukemic cells.; Authors - Wei Lu
mouse	IF	Andrology; Exposure to elevated temperature affects the expression of piwi-interacting RNAs and associated transcripts in mouse testes; Authors - Wenbin Chen
rat	IHC	World J Gastroenterol; Hydrogen-rich water alleviates constipation by attenuating oxidative stress through the sirtuin1/nuclear factor-erythroid-2-related factor 2/heme oxygenase-1 signaling pathway; Authors - Kai-Di Chen

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