KIF6 Polyclonal antibody

KIF6 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 17290-1-AP

Synonyms

C6orf102, KIF6, kinesin family member 6, Kinesin like protein KIF6

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Validation Data Gallery

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  WB analysis using 17290-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17290-1-AP (KIF6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 17290-1-AP

  IP result of anti-KIF6 (IP:17290-1-AP, 4ug; Detection:17290-1-AP 1:500) with mouse testis tissue lysate 4000ug.

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  IHC staining of human liver using 17290-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17290-1-AP (KIF6 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 17290-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17290-1-AP (KIF6 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse testis tissue, rat testis tissue
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 2 publications below

Product Information

17290-1-AP targets KIF6 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KIF6 fusion protein Ag11164
• Full Name: kinesin family member 6
• Calculated Molecular Weight: 265aa,30 kDa; 814aa,93 kDa
• Observed Molecular Weight: 93 kDa
• GenBank Accession Number: BC022074
• Gene Symbol: KIF6
• Gene ID (NCBI): 221458
• RRID: AB_2296657
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse,monkey	WB,IF	bioRxiv; Kif6 regulates cilia motility and polarity in brain ependymal cells; Authors - Maki Takagishi
mouse	IF,WB	Dis Model Mech; Motor protein Kif6 regulates cilia motility and polarity in brain ependymal cells; Authors - Maki Takagishi