KIF5C Polyclonal antibody

• Featured Product
• KD/KO Validated

KIF5C Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

9

Reviews (1)

Cat no : 25897-1-AP

Synonyms

NKHC, KINN, Kinesin-1, Kinesin heavy chain neuron-specific 2, KIAA0531

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Validation Data Gallery

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  WB analysis using 25897-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25897-1-AP (KIF5C antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 25897-1-AP

  IP result of anti-KIF5C (IP:25897-1-AP, 4ug; Detection:25897-1-AP 1:800) with mouse brain tissue lysate 2080 ug.

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  IHC staining of mouse brain using 25897-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25897-1-AP (KIF5C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 25897-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25897-1-AP (KIF5C antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of SH-SY5Y using 25897-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using KIF5C antibody (25897-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of HeLa using 25897-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using KIF5C antibody (25897-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells, SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 1 publications below
• CoIP: See 1 publications below

Product Information

25897-1-AP targets KIF5C in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KIF5C fusion protein Ag23163
• Full Name: kinesin family member 5C
• Calculated Molecular Weight: 725 aa, 83 kDa
• Observed Molecular Weight: 70 kDa, 110 kDa
• GenBank Accession Number: BC110287
• Gene Symbol: KIF5C
• Gene ID (NCBI): 3800
• RRID: AB_2880288
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KIF5C (Kinesin heavy chain isoform 5C variant) which belongs to the kinesin-like protein family is neuron specific. The kinesin-1 subfamilies (kif5a, kif5b, kif5c) are responsible for the movement of mitochondria in neurons. Kinesins have N-terminal motor domains and C-terminal cargo-binding tail domains separated by hinge regions. The hinge and C-terminal tail regions of Kif5a, Kif5b, and Kif5c bound a large detergent-resistant RNase-sensitive granule. The granule localized to dendrites and underwent bidirectional movement. Distally directed movement of the granule was enhanced by Kif5 overexpression and reduced by Kif5 functional blockage. kinesins may transport RNA in dendrites via this large granule. Two major isoforms of kif5c, with molecular predicted masses of 80 and 110 kDa, are generated by alternative splicing.

Publications

Species	Application	Title
human	WB	iScience; MFN2 coordinates mitochondria motility with α-tubulin acetylation and this regulation is disrupted in CMT2A; Authors - Atul Kumar
mouse	WB	Exp Neurol; Axonal injury mediated by neuronal p75NTR/TRAF6/JNK pathway contributes to cognitive impairment after repetitive mTBI; Authors - Gang Liu
human	WB	Hum Mol Genet; Mitochondrial transport mediates survival of retinal ganglion cells in affected LHON patients.; Authors - Tien-Chun Yang
mouse	WB	Alzheimers Res Ther; Normal levels of KIF5 but reduced KLC1 levels in both Alzheimer disease and Alzheimer disease in Down syndrome: evidence suggesting defects in anterograde transport.; Authors - Xu-Qiao Chen
human	WB	bioRxiv; MFN2-dependent recruitment of ATAT1 coordinates mitochondria motility with alpha-tubulin acetylation and is disrupted in CMT2A; Authors - Atul Kumar
human	WB,IHC	Int Endod J; Overexpression of MicroRNA-155 Aggravates Pulpitis by Targeting Kinesin Superfamily Proteins-5C Based on Illumina High-Throughput Sequencing; Authors - Ye Jiang ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Xuqiao (Verified Customer) (08-31-2020)

It can produce a clear band at 120-130kD which matches it mw, although it also has some uncharacteristic bands below 100 kD.

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: mouse brain

KIF5C Antibody Western Blot validation (1:3000 dilution) in mouse brain (Cat no:25897-1-AP)