KIF5B Polyclonal antibody

• Featured Product
• KD/KO Validated

KIF5B Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

26

Reviews (3)

Cat no : 21632-1-AP

Synonyms

KIF5B, Kinesin 1 heavy chain, kinesin family member 5B, KINH, KNS, KNS1, Ubiquitous kinesin heavy chain, UKHC

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Validation Data Gallery

• 

  WB analysis of A549 using 21632-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21632-1-AP (KIF5B antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 21632-1-AP

  WB result of KIF5B antibody (21632-1-AP; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KIF5B transfected HeLa cells.

• 

  IP experiment of HepG2 using 21632-1-AP

  IP result of anti-KIF5B (IP:21632-1-AP, 5ug; Detection:21632-1-AP 1:2000) with HepG2 cells lysate 3600ug.

• 

  IHC staining of human kidney using 21632-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 21632-1-AP (KIF5B antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 21632-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 21632-1-AP (KIF5B antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human skin using 21632-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 21632-1-AP (KIF5B antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human skin using 21632-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 21632-1-AP (KIF5B antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 21632-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using KIF5B antibody (21632-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, HepG2 cells, Jurkat cells, mouse brain, mouse heart, rat brain
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human kidney tissue, human skin tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 6 publications below
• WB: See 22 publications below
• IHC: See 1 publications below
• IF: See 5 publications below
• IP: See 3 publications below
• CoIP: See 1 publications below

Product Information

21632-1-AP targets KIF5B in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KIF5B fusion protein Ag16250
• Full Name: kinesin family member 5B
• Calculated Molecular Weight: 963 aa, 110 kDa
• Observed Molecular Weight: 110-120 kDa
• GenBank Accession Number: BC126279
• Gene Symbol: KIF5B
• Gene ID (NCBI): 3799
• RRID: AB_11182931
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KIF5B, also known as KINH, KNS1, belongs to the Kinesin-1 family. KIF5B contains three structural domains: a globular N-terminal motor domain, a central alpha-helical rod domain, and a globular C-terminal domain. During the G2 phase of the cell cycle in a BICD2-dependent manner, KIF5B antagonizes the dynein function and drives the separation of nuclei and centrosomes (PMID: 20386726). KIF5B drives the polarization of cytolytic granules and microtubule-organizing centers (MTOCs) toward the immune synapse between effector NK lymphocytes and target cells (KIF5B:24088571).

Publications

Species	Application	Title
human	WB,IF,IP	Arterioscler Thromb Vasc Biol; Kinesin-1: A New Actor Involved in Platelet Secretion and Thrombus Stability.; Authors - Frédéric Adam ; KO Validated
rat	IP	J Hazard Mater; Nano-graphene oxide depresses neurotransmission by blocking retrograde transport of mitochondria.; Authors - Yiyuan Kang
mouse	WB,IF	J Cell Biol; Kinesin-1 controls mast cell degranulation and anaphylaxis through PI3K-dependent recruitment to the granular Slp3/Rab27b complex.; Authors - Isabelle Munoz ; KO Validated
human	WB	EMBO Rep; MICAL2PV suppresses the formation of tunneling nanotubes and modulates mitochondrial trafficking.; Authors - Fei Wang
mouse	IF	Elife; Brucella activates the host RIDD pathway to subvert BLOS1-directed immune defense.; Authors - Kelsey Michelle Wells
human	WB	Cancers (Basel); GDF15 Is an Eribulin Response Biomarker also Required for Survival of DTP Breast Cancer Cells.; Authors - Chiara Bellio

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Susana (Verified Customer) (11-30-2022)

The antibody works very well on western blotting. I did an incubation at room temperature for 2 hours and it worked perfectly; I had one band at the expected molecular weight.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Retinoic-acid-induced differentiated SH-SY5Y cells

KIF5B Antibody Western Blot validation (1:1000 dilution) in Retinoic-acid-induced differentiated SH-SY5Y cells (Cat no:21632-1-AP)

FH

Xu (Verified Customer) (10-06-2020)

This one contains the image. I specifically probes KIF5B from both mouse brain and PC12/HEK293T cells. It can also be used in IP with a high titer. Awesome!

• Applications: Western Blot, Immunoprecipitation
• Primary Antibody Dilution: 1:3000 WB; 1 ug/mg total protein IP
• Cell Tissue Type: mouse brain, PC12 cell

KIF5B Antibody Western Blot,Immunoprecipitation validation (1:3000 WB; 1 ug/mg total protein IP dilution) in mouse brain, PC12 cell (Cat no:21632-1-AP)

FH

D (Verified Customer) (09-26-2019)

Works excellent in western blots and immunoprecipitations. Good antibody concentration for doing multiple experiments.

• Applications: Western Blot, Immunoprecipitation,
• Primary Antibody Dilution: 1:4000 (WB), 5ug/mg (IP)
• Cell Tissue Type: Endothelial cells

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