KIF5A Recombinant antibody, PBS Only (Capture)

KIF5A Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241124H1

Cat no : 84013-4-PBS

Synonyms

Neuronal kinesin heavy chain, MY050, Kinesin heavy chain neuron-specific 1, Kinesin heavy chain isoform 5A, D12S1889

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Validation Data Gallery

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  WB analysis of rat brain using 84013-4-RR (same clone as 84013-4-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 84013-4-RR (KIF5A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84013-4-PBS in a different storage buffer formulation.

• 

  IF Staining of PC-12 using 84013-4-RR (same clone as 84013-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using KIF5A antibody (84013-4-RR, Clone: 241124H1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84013-4-PBS in a different storage buffer formulation.

• 

  FC experiment of K-562 using 84013-4-RR (same clone as 84013-4-PBS)

  1x10^6 K-562 cells were intracellularly stained with 0.25 ug KIF5A Recombinant antibody (84013-4-RR, Clone:241124H1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84013-4-PBS in a different storage buffer formulation.

• 

  FC experiment of SH-SY5Y using 84013-4-RR (same clone as 84013-4-PBS)

  1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug KIF5A Recombinant antibody (84013-4-RR, Clone:241124H1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84013-4-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP00967-1

  Cytometric bead array standard curve of MP00967-1, KIF5A Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84013-4-PBS. Detection antibody: 84013-3-PBS. Standard: Ag15518. Range: 0.313-40 ng/mL.

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  Cytometric bead array standard curve of MP00967-2

  Cytometric bead array standard curve of MP00967-2, KIF5A Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84013-4-PBS. Detection antibody: 84013-1-PBS. Standard: Ag15518. Range: 0.313-40 ng/mL.

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  Affinity and Kinetic Characterization of 84013-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84013-4-RR against Human KIF5A were performed. The affinity constant is 78.9 pM.

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Product Information

84013-4-PBS targets KIF5A as part of a matched antibody pair:

MP00967-1: 84013-4-PBS capture and 84013-3-PBS detection (validated in Cytometric bead array)

MP00967-2: 84013-4-PBS capture and 84013-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: KIF5A fusion protein Ag15518
• Full Name: kinesin family member 5A
• Calculated Molecular Weight: 1032 aa, 117 kDa
• Observed Molecular Weight: 118-120 kDa
• GenBank Accession Number: BC146670
• Gene Symbol: KIF5A
• Gene ID (NCBI): 3798
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Kinesin superfamily proteins (KIFs) are microtubule-based molecular motors essential for the intracellular transport of various cargos, including organelles, proteins, and RNAs. KIF5A is expressed exclusively in neurons and transports neuronal cargoes into axons and dendrites. KIF5A mutations have been associated with Charcot-Marie-Tooth Type 2, an axonal peripheral neuropathy characterized by progressive loss of peripheral sensation and muscle wasting.