KIF5A Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

KIF5A Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 21186-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 21186-1-AP (same clone as 21186-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21186-1-AP (KIF5A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21186-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 21186-1-AP (same clone as 21186-1-PBS)

  IP result of anti-KIF5A (IP:21186-1-AP, 4ug; Detection:21186-1-AP 1:2000) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 21186-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 21186-1-AP (same clone as 21186-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21186-1-AP (KIF5A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21186-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 21186-1-AP (same clone as 21186-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21186-1-AP (KIF5A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21186-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 21186-1-AP (same clone as 21186-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 21186-1-AP (KIF5A antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 21186-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 21186-1-AP (same clone as 21186-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 21186-1-AP (KIF5A antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 21186-1-PBS in a different storage buffer formulation.

• 

  IF Staining of PC-12 using 21186-1-AP (same clone as 21186-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using KIF5A antibody (21186-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 21186-1-PBS in a different storage buffer formulation.

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Product Information

21186-1-PBS targets KIF5A in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KIF5A fusion protein Ag15518
• Full Name: kinesin family member 5A
• Calculated Molecular Weight: 1032 aa, 117 kDa
• Observed Molecular Weight: 118-120 kDa
• GenBank Accession Number: BC146670
• Gene Symbol: KIF5A
• Gene ID (NCBI): 3798
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Kinesin superfamily proteins (KIFs) are microtubule-based molecular motors essential for the intracellular transport of various cargos, including organelles, proteins, and RNAs. KIF5A is expressed exclusively in neurons and transports neuronal cargoes into axons and dendrites. KIF5A mutations have been associated with Charcot-Marie-Tooth Type 2, an axonal peripheral neuropathy characterized by progressive loss of peripheral sensation and muscle wasting.