KIF26B Polyclonal antibody

• Featured Product
• KD/KO Validated

KIF26B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, Canine and More (2)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

13

Reviews (1)

Cat no : 17422-1-AP

Synonyms

KIF26B, kinesin family member 26B, Kinesin like protein KIF26B

---

Validation Data Gallery

• 

  WB analysis of MDCK using 17422-1-AP

  MDCK cells were subjected to SDS PAGE followed by western blot with 17422-1-AP (KIF26B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 17422-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17422-1-AP (KIF26B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 17422-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 17422-1-AP (KIF26B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 17422-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17422-1-AP (KIF26B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 17422-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17422-1-AP (KIF26B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 17422-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17422-1-AP (KIF26B Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human breast cancer using 17422-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17422-1-AP (KIF26B Antibody) at dilution of 1:200 (under 40x lens).

---

Tested Applications

• Positive WB detected in: MDCK cells, HEK-293 cells, MCF-7 cells
• Positive IHC detected in: human kidney tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 6 publications below
• WB: See 8 publications below
• IHC: See 7 publications below
• IF: See 3 publications below

Product Information

17422-1-AP targets KIF26B in WB, IF, IHC, ELISA applications and shows reactivity with human, Canine samples.

• Tested Reactivity: human, Canine
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KIF26B fusion protein Ag11446
• Full Name: kinesin family member 26B
• Calculated Molecular Weight: 2108aa,224 kDa; 162aa,17 kDa
• Observed Molecular Weight: 224-230 kDa, 17-22 kDa
• GenBank Accession Number: BC042481
• Gene Symbol: KIF26B
• Gene ID (NCBI): 55083
• RRID: AB_2131762
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KIF26B is a member of kinesin-11 family and plays important role in embryogenesis. It is a downstram target of Sall1 and is essential for kidney development. KIF26B is also involved in tumorigenesis.

Publications

Species	Application	Title
human,mouse	WB,IHC	Nat Commun; Fusobacterium nucleatum reduces METTL3-mediated m6A modification and contributes to colorectal cancer metastasis.; Authors - Shujie Chen ; KD Validated
human	IHC,WB	Biomed Pharmacother; KIF26B promotes cell proliferation and migration through the FGF2/ERK signaling pathway in breast cancer.; Authors - Yan Teng
human,mouse	WB,IF	J Bone Miner Res; KIF26B Silencing Prevents Osseous Transdifferentiation of Progenitor/Stem Cells and Attenuates Ectopic Calcification in a Murine Model.; Authors - Mingming Yan ; KD Validated
human	IF	J Ginseng Res; Ginsenoside Rh2 reduces m6A RNA methylation in cancer via the KIF26B-SRF positive feedback loop; Authors - Chunmei Hu
human	IHC	Cell Cycle; Noncoding RNAs-based high KIF26B expression correlates with poor prognosis and tumor immune infiltration in colon cancer; Authors - Zhihong Liu
mouse	IF	Biochem Biophys Res Commun; Novel mechanistic role of Kif26b in adipogenic differentiation of murine multipotent stromal cells.; Authors - Mingming Yan ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Abigail (Verified Customer) (06-17-2024)

We had a lot of issues with this antibody due to difficulties transferring the high molecular weight protein. When we were able to get a good transfer as seen on the Ponceau, it was pretty hit or miss on if the antibody worked. We only had one quantifiable blot, the rest were not able to be transferred due to poor signal.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Brain cortex