KIF22 Polyclonal antibody

• Featured Product
• KD/KO Validated

KIF22 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 13403-1-AP

Synonyms

Kinesin-like protein KIF22, Kinesin-like protein 4, Kinesin-like DNA-binding protein, Kinesin like protein KIF22, Kinesin like protein 4

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Validation Data Gallery

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  WB analysis using 13403-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13403-1-AP (KIF22 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 13403-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 13403-1-AP (KIF22 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 13403-1-AP

  IP result of anti-KIF22 (IP:13403-1-AP, 4ug; Detection:13403-1-AP 1:300) with HeLa cells lysate 4000ug.

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  IHC staining of human brown disease using 13403-1-AP

  Immunohistochemical analysis of paraffin-embedded human brown disease slide using 13403-1-AP (KIF22 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 13403-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13403-1-AP (KIF22 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 13403-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13403-1-AP (KIF22 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 13403-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13403-1-AP (KIF22 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 13403-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13403-1-AP (KIF22 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 13403-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13403-1-AP (KIF22 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 13403-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13403-1-AP (KIF22 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 13403-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13403-1-AP (KIF22 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 13403-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13403-1-AP (KIF22 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Jurkat cells, human heart tissue, MKN-45 cells, T-47D cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human stomach cancer tissue, human breast cancer tissue, human brown disease, human cervical cancer tissue, mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

13403-1-AP targets KIF22 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KIF22 fusion protein Ag4254
• Full Name: kinesin family member 22
• Calculated Molecular Weight: 665 aa, 73 kDa
• Observed Molecular Weight: 68-73 kDa
• GenBank Accession Number: BC028155
• Gene Symbol: KIF22
• Gene ID (NCBI): 3835
• RRID: AB_2249576
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KIF22, also known as KID (kinesin-like DNA-binding protein), is a kinesin-like DNA-binding protein known to be involved in chromosome movement during mitosis. KIF22 is a nuclear protein regulated by SIAH-1, whose level fluctuate in a cell cycle-dependent manner, increasing during pre-mitotic phases and greatly decreasing during mitosis. KIF22 localizes to the nucleus of the interphase cells upon entry into mitosis and displays some punctate cytoplasmic staining. Defects in KIF22 are the cause of spondyloepimetaphyseal dysplasia with joint laxity, type 2 (SEMDJL2).

Publications

Species	Application	Title
mouse	IF	Sci Rep; PMID: 29229923; Authors - Sara Bizzotto
human	WB	Proteomics; Genistein-induced mitotic arrest of gastric cancer cells by downregulating KIF20A, a proteomics study.; Authors - Yan Guang-Rong GR
human	WB,IHC	Chin Med J (Engl); Effect of KIF22 on promoting proliferation and migration of gastric cancer cells via MAPK-ERK pathways.; Authors - Ze-Yuan Yu ; KD Validated