CoraLite®594-conjugated KIF20A Monoclonal antibody

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  IF Staining of HeLa using CL594-67190

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594-conjugated KIF20A antibody (CL594-67190, Clone: 2A8E3 ) at dilution of 1:200, CL488-Phalloidin (green).

• 

  IF Staining of HeLa using CL594-67190

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CL594-67190 (KIF20A antibody) at dilution of 1:100.

• Featured Product
• KD/KO Validated

KIF20A Monoclonal Antibody for IF/ICC

Cat No. CL594-67190

Clone No.2A8E3

Publications(1)

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Mouse

Applications

IF/ICC

Synonyms

FLJ21151, GG10_2, KIF20A, kinesin family member 20A, Kinesin like protein KIF20A, MKLP2, RAB6KIFL, Rabkinesin 6

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Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

CL594-67190 targets KIF20A in IF/ICC applications and shows reactivity with Human, Rat, Mouse samples.

• Tested Reactivity: Human, Rat, Mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: KIF20A fusion protein Ag8916
• Full Name: kinesin family member 20A
• Calculated Molecular Weight: 890 aa, 100 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC012999
• Gene Symbol: KIF20A
• Gene ID (NCBI): 10112
• RRID: AB_2883645
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: O95235
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

KIF20A, also known as RAB6KIFL or MKlp2, is a kinesin originally identified as a binding partner for the rab6 GTPase involved in protein transport at the Golgi apparatus. KIF20A belongs to a large family of motor proteins that accumulate in mitotic cells and is required for normal cell division. KIF20A is thought to be involved in carcinogenesis. In normal tissues KIF20A is almost undetectable while it is overexpressed in various cancers. KIF20A has been identified as a novel promising candidate for anticancer immunotherapy.

Publications

Species	Application	Title
	IF	Science; The mechanism of acentrosomal spindle assembly in human oocytes; Authors - Tianyu Wu