KIF20A Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

KIF20A Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2A8E3

Cat no : 67190-1-PBS

Synonyms

MKlp2, Mitotic kinesin-like protein 2, Kinesin-like protein KIF20A, GG10_2, FLJ21151

---

Validation Data Gallery

• 

  WB analysis using 67190-1-Ig (same clone as 67190-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67190-1-Ig (KIF20A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 67190-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 67190-1-Ig (same clone as 67190-1-PBS)

  WB result of KIF20A antibody (67190-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KIF20A transfected HeLa cells. This data was developed using the same antibody clone with 67190-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67190-1-Ig (same clone as 67190-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67190-1-Ig (KIF20A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67190-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 67190-1-Ig (same clone as 67190-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67190-1-Ig (KIF20A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67190-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 67190-1-Ig (same clone as 67190-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67190-1-Ig (KIF20A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67190-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 67190-1-Ig (same clone as 67190-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67190-1-Ig (KIF20A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67190-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 67190-1-Ig (same clone as 67190-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67190-1-Ig (KIF20A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67190-1-PBS in a different storage buffer formulation.

---

Product Information

67190-1-PBS targets KIF20A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: KIF20A fusion protein Ag8916
• Full Name: kinesin family member 20A
• Calculated Molecular Weight: 890 aa, 100 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC012999
• Gene Symbol: KIF20A
• Gene ID (NCBI): 10112
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

KIF20A, also known as RAB6KIFL or MKlp2, is a kinesin originally identified as a binding partner for the rab6 GTPase involved in protein transport at the Golgi apparatus. KIF20A belongs to a large family of motor proteins that accumulate in mitotic cells and is required for normal cell division. KIF20A is thought to be involved in carcinogenesis. In normal tissues KIF20A is almost undetectable while it is overexpressed in various cancers. KIF20A has been identified as a novel promising candidate for anticancer immunotherapy.