KIF18A Polyclonal antibody

• Featured Product
• KD/KO Validated

KIF18A Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

13

Cat no : 19245-1-AP

Synonyms

PPP1R99, OK/SW-cl.108, MS-KIF18A, Kinesin-like protein KIF18A

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Validation Data Gallery

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  WB analysis of Jurkat using 19245-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19245-1-AP (KIF18A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 19245-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19245-1-AP (KIF18A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 19245-1-AP

  IP result of anti-KIF18A (IP:19245-1-AP, 4ug; Detection:19245-1-AP 1:500) with Jurkat cells lysate 2400ug.

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  IHC staining of human colon cancer using 19245-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 19245-1-AP (KIF18A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 19245-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 19245-1-AP (KIF18A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 19245-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19245-1-AP (KIF18A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 19245-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19245-1-AP (KIF18A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 19245-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19245-1-AP (KIF18A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 19245-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19245-1-AP (KIF18A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human colon cancer, human breast cancer tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 8 publications below
• IHC: See 8 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Product Information

19245-1-AP targets KIF18A in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KIF18A fusion protein Ag6166
• Full Name: kinesin family member 18A
• Calculated Molecular Weight: 102 kDa
• Observed Molecular Weight: 102 kDa
• GenBank Accession Number: BC048347
• Gene Symbol: KIF18A
• Gene ID (NCBI): 81930
• RRID: AB_10598464
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KIF18A belongs to the Kinesin family. KIF18A binds to microtubules and moves along them using energy from ATP hydrolysis. KIF18A is related to the localization of membrane organelles and protein complexes, as well as the maintenance of microtubule dynamics and mitosis. KIF18A expression is low in normal tissues and high in solid tumors, including breast cancer, hepatocellular carcinoma and lung adenocarcinoma, colorectal cancer, renal cell carcinoma, and head and neck squamous cell carcinoma. The molecular weight of KIF18A is 102 kDa (PMID: 36830695).

Publications

Species	Application	Title
human	IHC	Oncotarget; High KIF18A expression correlates with unfavorable prognosis in primary hepatocellular carcinoma.; Authors - Weijia Liao
human	WB	Oncotarget; Exclusive destruction of mitotic spindles in human cancer cells.; Authors - Leonid Visochek
mouse	IF	Sci Rep; PMID: 29229923; Authors - Sara Bizzotto
human	WB,IHC	Front Genet; Prognostic Biomarker KIF18A and Its Correlations With Immune Infiltrates and Mitosis in Glioma.; Authors - Bing-Yan Tao
human	WB	Dis Markers; Kinesin Family Member 18A (KIF18A) Contributes to the Proliferation, Migration, and Invasion of Lung Adenocarcinoma Cells In Vitro and In Vivo.; Authors - Fu-Tao Chen ; KD Validated
mouse	WB,IF	Cell Div; Kif18a regulates Sirt2-mediated tubulin acetylation for spindle organization during mouse oocyte meiosis.; Authors - Feng Tang ; KD Validated