KIF17 Polyclonal antibody

KIF17 Polyclonal Antibody for IF, IHC, IP,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 14615-1-AP

Synonyms

KIAA1405, KIF17, KIF17B, KIF3 related motor protein, KIF3X, kinesin family member 17, Kinesin like protein KIF17

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Validation Data Gallery

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  IP experiment of mouse brain using 14615-1-AP

  IP Result of anti-KIF17 (IP:14615-1-AP, 4ug; Detection:14615-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of human gliomas using 14615-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14615-1-AP (KIF17 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 14615-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14615-1-AP (KIF17 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 14615-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14615-1-AP (KIF17 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 14615-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14615-1-AP (KIF17 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 14615-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 14615-1-AP (KIF17 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 14615-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 14615-1-AP (KIF17 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of COS-7 using 14615-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed COS-7 cells using 14615-1-AP (KIF17 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human gliomas tissue, human brain tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: COS-7 cells

Recommended dilution

• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 1 publications below

Product Information

14615-1-AP targets KIF17 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

• Tested Reactivity: human, mouse, rat, monkey
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KIF17 fusion protein Ag6217
• Full Name: kinesin family member 17
• Calculated molecular weight: 115 kDa
• Observed molecular weight: 170 kDa
• GenBank accession number: BC065927
• Gene symbol: KIF17
• Gene ID (NCBI): 57576
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Publications

Species	Application	Title
mouse	WB	Dev Cell; ASK1-Mediated Phosphorylation Blocks HDAC6 Ubiquitination and Degradation to Drive the Disassembly of Photoreceptor Connecting Cilia.; Authors - Jie Ran