KIF15 Recombinant antibody

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  WB analysis using 82903-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82903-1-RR (KIF15 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human intrahepatic cholangiocarcinoma using 82903-1-RR

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82903-1-RR (KIF15 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 82903-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82903-1-RR against Human KIF15 were performed. The affinity constant is 75.8 pM.

KIF15 {ptg:Brand} Recombinant Antibody for WB, IHC, ELISA

Cat No. 82903-1-RR

Clone No.230049F1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

KLP2, Kinesin-like protein KIF15, Kinesin-like protein 7, Kinesin-like protein 2, hKLP2

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, HepG2 cells, K-562 cells
• Positive IHC detected in: human intrahepatic cholangiocarcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82903-1-RR targets KIF15 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: KIF15 fusion protein Ag33897
• Full Name: kinesin family member 15
• Calculated Molecular Weight: 160 kDa
• Observed Molecular Weight: 160 kDa
• GenBank Accession Number: NM_020242
• Gene Symbol: KIF15
• Gene ID (NCBI): 56992
• RRID: AB_3670632
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9NS87
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KIF15, also named as KLP2 and KNSL7, belongs to the kinesin-like protein family and KLP2 subfamily. It is a plus-end directed kinesin-like motor enzyme which involved in mitotic spindle assembly. Several isoforms of KIF15 exist due to the alternative splicing, with molecular weight between 130-160 kDa. Sometimes higher molecular weight around 200 kDa can also be observed, which may be a modified variant of KIF15. (14618103)