KIF15 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

KIF15 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 55407-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 55407-1-AP (same clone as 55407-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 55407-1-AP (KIF15 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55407-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 55407-1-AP (same clone as 55407-1-PBS)

  WB result of KIF15 antibody (55407-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KIF15 transfected HEK-293 cells. This data was developed using the same antibody clone with 55407-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 55407-1-AP (same clone as 55407-1-PBS)

  WB result of KIF15 antibody (55407-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KIF15 transfected Jurkat cells. This data was developed using the same antibody clone with 55407-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse thymus using 55407-1-AP (same clone as 55407-1-PBS)

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 55407-1-AP (KIF15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55407-1-PBS in a different storage buffer formulation.

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  WB analysis of MDA-MB-453s using 55407-1-AP (same clone as 55407-1-PBS)

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 55407-1-AP (KIF15 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55407-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse thymus using 55407-1-AP (same clone as 55407-1-PBS)

  IP result of anti-KIF15 (IP:55407-1-AP, 4ug; Detection:55407-1-AP 1:500) with mouse thymus tissue lysate 2800ug. This data was developed using the same antibody clone with 55407-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 55407-1-AP (same clone as 55407-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 55407-1-AP (KIF15 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 55407-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 55407-1-AP (same clone as 55407-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 55407-1-AP (KIF15 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 55407-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 55407-1-AP (same clone as 55407-1-PBS)

  Immunofluorescent analysis of HEK-293 cells using 55407-1-AP (KIF15 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 55407-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HEK-293 using 55407-1-AP (same clone as 55407-1-PBS)

  1x10^6 HEK-293 cells were intracellularly stained with 0.4 ug KIF15 Polyclonal antibody (55407-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 55407-1-PBS in a different storage buffer formulation.

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Product Information

55407-1-PBS targets KIF15 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: kinesin family member 15
• Calculated Molecular Weight: 160 kDa
• Observed Molecular Weight: 150-165 kDa
• GenBank Accession Number: NM_020242
• Gene Symbol: KIF15
• Gene ID (NCBI): 56992
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

KIF15, also named as KLP2 and KNSL7, belongs to the kinesin-like protein family and KLP2 subfamily. It is a plus-end directed kinesin-like motor enzyme which involved in mitotic spindle assembly. Several isoforms of KIF15 exist due to the alternative splicing, with molecular weight between 130-160 kDa. Sometimes higher molecular weight around 200 kDa can also be observed, which may be a modified variant of KIF15. (14618103)