KIF15 Polyclonal antibody

• 

  WB analysis using 30774-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30774-1-AP (KIF15 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

KIF15 Polyclonal Antibody for WB, ELISA

Cat No. 30774-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Synonyms

FLJ25667, HKLP2, KIF15, kinesin family member 15, Kinesin like protein 2, Kinesin like protein 7, Kinesin like protein KIF15, KLP2, KNSL7, NY BR 62

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, HepG2 cels, K-562 cells, L02 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30774-1-AP targets KIF15 in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KIF15 fusion protein Ag33897
• Full Name: kinesin family member 15
• Calculated Molecular Weight: 160 kDa
• Observed Molecular Weight: 145-150 kDa
• GenBank Accession Number: NM_020242
• Gene Symbol: KIF15
• Gene ID (NCBI): 56992
• RRID: AB_3086416
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NS87
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KIF15, also named as KLP2 and KNSL7, belongs to the kinesin-like protein family and KLP2 subfamily. It is a plus-end directed kinesin-like motor enzyme which involved in mitotic spindle assembly. It is located in the cytoplasm and biased expression in testis. Several isoforms of KIF15 exist due to the alternative splicing, with molecular weight between 130-160 kDa. Sometimes higher molecular weight around 200 kDa can also be observed, which may be a modified variant of KIF15 (14618103). It is known that KIF15 participates in important events during neuronal development and involves multiple cellular processes such as proliferation, apoptosis, and differentiation. There is emerging evidence indicating that KIF15 plays an important role in several malignancies including pancreatic cancer, hepatocellular carcinoma, and breast cancer (PMID: 33277366).