KIAA2013 Polyclonal antibody

KIAA2013 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27886-1-AP

Synonyms

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Validation Data Gallery

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  WB analysis of HEK-293 using 27886-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 27886-1-AP (KIAA2013 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 27886-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27886-1-AP (KIAA2013 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U2OS using 27886-1-AP

  U2OS cells were subjected to SDS PAGE followed by western blot with 27886-1-AP (KIAA2013 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 27886-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 27886-1-AP (KIAA2013 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 27886-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 27886-1-AP (KIAA2013 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, U2OS cells, mouse kidney tissue
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27886-1-AP targets KIAA2013 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KIAA2013 fusion protein Ag27379
• Full Name: KIAA2013
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC035033
• Gene Symbol: KIAA2013
• Gene ID (NCBI): 90231
• RRID: AB_2881004
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KIAA2013, also known as Q8IYS2 or MGC33867, is a single-pass transmembrane protein encoded by the KIAA2013 gene in humans. KIAA2013 has been found to play a role in the Endocannabinoid system. This system is made up of cannabinoid receptors 1 and 2 (CB1 and CB2) as well as the various ligands and enzymes that interact. The protein KIAA2013 has been found to be expressed within CB2 expressing cells. Both cannabinoid receptors are labeled as class A G-Protein Coupled Receptors, and CB2 is highly expressed within the human spleen and leukocytes. CB2, and by extension KIAA2013, are therefore targets of interest for therapeutic studies looking into diseases such as inflammatory bowel disease and rheumatoid arthritis(PMID:31616248 34752341).