KIAA1715 Recombinant antibody

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  WB analysis using 83418-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83418-5-RR (KIAA1715 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse cerebellum using 83418-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83418-5-RR (KIAA1715 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U-251 using 83418-5-RR

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using KIAA1715 antibody (83418-5-RR, Clone: 240211B9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

KIAA1715 {ptg:Brand} Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 83418-5-RR

Clone No.240211B9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

LNP, Ul, Protein lunapark, LNPK, ER junction formation factor lunapark

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Tested Applications

• Positive WB detected in: HEK-293 cells, K-562 cells, HepG2 cells, U2OS cells, mouse testis tissue, mouse colon tissue, mouse kidney tissue
• Positive IHC detected in: mouse cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U-251 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83418-5-RR targets KIAA1715 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: KIAA1715 fusion protein Ag34669
• Full Name: KIAA1715
• Observed Molecular Weight: 53-55 kDa
• GenBank Accession Number: BC105132
• Gene Symbol: KIAA1715
• Gene ID (NCBI): 80856
• RRID: AB_3671067
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9C0E8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KIAA1715, also known as LNPK, is a member of the conserved Lunapark protein family. KIAA1715 is an endoplasmic reticulum (ER) membrane protein consisting of an N-terminal domain important for N-myristoylation and ER targeting, two transmembrane domains and a unique zinc finger domain. It has 4 isoforms produced by alternative splicing and acts mainly as a stabilizer of negative membrane curvature (PMID: 27619977).