VIRMA/KIAA1429 Polyclonal antibody

• Featured Product
• KD/KO Validated

VIRMA/KIAA1429 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 30166-1-AP

Synonyms

KIAA1429, VIRMA, MSTP054

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Validation Data Gallery

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  WB analysis using 30166-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30166-1-AP (KIAA1429 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 30166-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 30166-1-AP (VIRMA/KIAA1429 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 30166-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using VIRMA/KIAA1429 antibody (30166-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Tested Applications

• Positive WB detected in: A431 cells, HeLa cells, K-562 cells, MDA-MB-231 cells, SKOV-3 cells
• Positive IHC detected in: mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

30166-1-AP targets VIRMA/KIAA1429 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: VIRMA/KIAA1429 fusion protein Ag32830
• Full Name: KIAA1429
• Observed Molecular Weight: 200-210 kDa
• GenBank Accession Number: BC003701
• Gene Symbol: KIAA1429
• Gene ID (NCBI): 25962
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VIRMA (KIAA1429) is a key component of m6A methyltransferase (writer) complex that include METTL3, METTL14, WTAP, VIRMA, HAKAI, ZC3H13, and RBM15. VIRMA mediates methylation in the 3'UTR and around the stop codon, thus affecting alternative polyadenylation. VIRMA has been reported to act as an oncogenic factor in breast cancer and liver cancer. Moreover, this antibody can not recognize short isoforms.

Publications

Species	Application	Title
human	WB	Cancer Lett; KIAA1429 facilitates metastasis via m6A-YTHDC1-dependent RND3 down-regulation in hepatocellular carcinoma cells; Authors - Meihua Shan ; KD Validated