TALPID3 Polyclonal antibody

• Featured Product
• KD/KO Validated

TALPID3 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, canine and More (1)

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugate

Unconjugated

Publications

11

Reviews (1)

Cat no : 24421-1-AP

Synonyms

KIAA0586, KIAA0586 isoform, Talpid3

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Validation Data Gallery

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  IHC staining of human brain using 24421-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24421-1-AP (TALPID3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 24421-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24421-1-AP (TALPID3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MDCK using 24421-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MDCK cells using TALPID3 antibody (24421-1-AP) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MDCK cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 4 publications below
• IF: See 7 publications below
• CoIP: See 2 publications below

Product Information

24421-1-AP targets TALPID3 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, canine samples.

• Tested Reactivity: human, mouse, canine
• Cited Reactivity: human, mouse, caenorhabditis elegans
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TALPID3 fusion protein Ag19813
• Full Name: KIAA0586
• Calculated Molecular Weight: 1533 aa, 169 kDa
• GenBank Accession Number: BC066647
• Gene Symbol: TALPID3
• Gene ID (NCBI): 9786
• RRID: AB_2879539
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KIAA0586 also named as Talpid3 is a 1472 amino-acid protein, which belongs to Talpid3 family. Talpid3 is a gene essential for vertebrate development. Studies in chicken, mouse, zebrafish and human have shown that TALPID3 is a centrosomal protein, mutants null for TALPID3 fail to produce cilia, resulting in far reaching signalling defects such as polydactyly, dorsalised neural tube, craniofacial and vascular defects often due to abnormalities in Hedgehog signal transduction. Talpid3 is absolutely required for the function of both Gli repressor and activator in the intracellular Hedgehog pathway and ciliogenesis. TALPID3 may be identified as a subcellular maker of centrosome and needed to further studied.

Publications

Species	Application	Title
human,Caenorhabditis elegans	WB,IF,CoIP	Nat Commun; TALPID3 and ANKRD26 selectively orchestrate FBF1 localization and cilia gating.; Authors - Hao Yan ; KD Validated
mouse	IF	Elife; TALPID3 controls centrosome and cell polarity and the human ortholog KIAA0586 is mutated in Joubert syndrome (JBTS23).; Authors - Louise A Stephen
human	IF	J Cell Biol; A ciliopathy complex builds distal appendages to initiate ciliogenesis.; Authors - Dhivya Kumar
human	IF	Elife; Centriolar satellites expedite mother centriole remodeling to promote ciliogenesis; Authors - Emma A Hall
human	WB	EMBO Rep; Aurora Kinase A proximity map reveals centriolar satellites as regulators of its ciliary function.; Authors - Melis D Arslanhan
mouse		Development; Centrosomal protein CP110 controls maturation of mother centriole during cilia biogenesis.; Authors - Sharda Prasad Yadav

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Elisa (Verified Customer) (12-14-2022)

RPE1 cells were fixed in cold methanol for 10' at -20C. Cells were then rehydrated with PBS for 5'. Membrane permeabilization was then performed with 0.1% Triton + 0.1% Tween +0.01%SDS in PBS for 5'. Cells were finally incubated with blocking buffer (5% BSA+ 0.1% Tween in PBS) for 30' at RT. Primary antibody was diluted in blocking buffer 1:200 and incubated for 1h at room temperature. Alexa-488-Anti-rabbit was used as secondary antibody for Talpid3 (1:600 dilution) (1h at room temperature). In pink (Talpid3); in green (GT335, decorating centrioles) and in blue (Hoechst, DNA).

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: RPE1 cells

TALPID3 Antibody Immunofluorescence validation (1:200 dilution) in RPE1 cells (Cat no:24421-1-AP)