KI67 Polyclonal antibody

KI67 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

45

Cat no : 19972-1-AP

Synonyms

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Validation Data Gallery

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  IHC staining of human breast cancer using 19972-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19972-1-AP (KI67 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

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  IHC staining of human breast cancer using 19972-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19972-1-AP (KI67 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

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Tested Applications

• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 36 publications below
• IF: See 1 publications below

Product Information

19972-1-AP targets KI67 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: antigen identified by monoclonal antibody Ki-67
• Calculated Molecular Weight: 359 kDa
• GenBank Accession Number: NM_002417
• Gene Symbol: KI67
• Gene ID (NCBI): 4288
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MKI67 gene encodes a nuclear protein, antigen KI-67, which was identified by monoclonal antibody Ki-67. Expression of this antigen occurs preferentially during late G1, S, G2 and M phases of the cell cycle, while in cells in G0 phase the antigen cannot be detected (PMID: 8227122). The antigen Ki-67 is a human nuclear protein the expression of which is strictly associated with cell proliferation and which is widely used in routine pathology as a "proliferation marker" to measure the growth fraction of cells in human tumors (PMID: 8227122).

Publications

Species	Application	Title
mouse	IHC	Phytomedicine; Ginsenoside Rg5 inhibits glioblastoma by activating ferroptosis via NR3C1/HSPB1/NCOA4; Authors - Guoqing Zhang
human	IHC	J Invest Dermatol; FUT8 remodeling of EGFR regulates epidermal keratinocyte proliferation during psoriasis development.; Authors - Musin Kelel
human	IHC	Oncotarget; miR-16 targets fibroblast growth factor 2 to inhibit NPC cell proliferation and invasion via PI3K/AKT and MAPK signaling pathways.; Authors - Qingmei He
mouse	IHC	Int J Cancer; Inhibition of tumor growth by U0126 is associated with induction of interferon-γ production.; Authors - Xingzhe Ma
human	IHC	Acta Biomater; Systematic evaluation of multifunctional paclitaxel-loaded polymeric mixed micelles as a potential anticancer remedy to overcome multidrug resistance.; Authors - Jiulong Zhang
human	IHC	Sci Rep; PMID: 26487539; Authors - Yuan Zhang