KHSRP Recombinant antibody, PBS Only

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  IP experiment of HeLa using 82931-2-RR (same clone as 82931-2-PBS)

  IP result of anti-KHSRP (IP:82931-2-RR, 4ug; Detection:82931-2-RR 1:2500) with HeLa cells lysate 1050 ug. This data was developed using the same antibody clone with 82931-2-PBS in a different storage buffer formulation.

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  FC experiment of U-251 using 82931-2-RR (same clone as 82931-2-PBS)

  1x10^6 U-251 cells were intracellularly stained with 0.25 ug Anti-Human KHSRP (82931-2-RR, Clone:230189C12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L) (red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 82931-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82931-2-RR

  Biolayer interferometry (BLl) kinetic assays of 82931-2-RR against Human KHSRP were performed. The affinity constant is 1.42 nM.

KHSRP Uni-rAb^TM Recombinant Antibody for IP, Indirect ELISA

Cat No. 82931-2-PBS

Clone No.230189C12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IP, Indirect ELISA

Synonyms

KH type-splicing regulatory protein, FUSE-binding protein 2, FUBP2, FBP2, Far upstream element-binding protein 2

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Product Information

82931-2-PBS targets KHSRP in IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: KHSRP fusion protein Ag33890
• Full Name: KH-type splicing regulatory protein
• Calculated Molecular Weight: 73 kDa
• GenBank Accession Number: NM_003685
• Gene Symbol: KHSRP
• Gene ID (NCBI): 8570
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q92945
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

KHSRP, also named as FUBP2, KSRP, and p75, belongs to the KHSRP family. It binds to the dendritic targeting element and may play a role in mRNA trafficking. KHSRP is a part of a ternary complex that binds to the downstream control sequence (DCS) of the pre-mRNA. It mediates exon inclusion in transcripts that are subject to tissue-specific alternative splicing. KHSRP may interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression. It is involved in the degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly by recruiting degradation machinery to ARE-containing mRNAs.