KHSRP Polyclonal antibody

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  WB analysis using 31044-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 31044-1-AP (KHSRP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 31044-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 31044-1-AP (KHSRP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 31044-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 31044-1-AP (KHSRP antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 31044-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using KHSRP antibody (31044-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

KHSRP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 31044-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

KSRP, KH type-splicing regulatory protein, FUSE-binding protein 2, FUBP2, FBP2

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Tested Applications

• Positive WB detected in: rat brain tissue
• Positive IHC detected in: human colon tissue, mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31044-1-AP targets KHSRP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KHSRP fusion protein Ag33890
• Full Name: KH-type splicing regulatory protein
• Calculated Molecular Weight: 73 kDa
• Observed Molecular Weight: 75-87kDa
• GenBank Accession Number: NM_003685
• Gene Symbol: KHSRP
• Gene ID (NCBI): 8570
• RRID: AB_3669829
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q92945
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.