KHK Polyclonal antibody

• Featured Product
• KD/KO Validated

KHK Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 15681-1-AP

Synonyms

Ketohexokinase, EC:2.7.1.3

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Validation Data Gallery

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  WB analysis using 15681-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15681-1-AP (KHK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 15681-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 15681-1-AP (KHK antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 15681-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15681-1-AP (KHK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 15681-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 15681-1-AP (KHK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 15681-1-AP

  IP result of anti-KHK (IP:15681-1-AP, 4ug; Detection:15681-1-AP 1:500) with mouse liver tissue lysate 4000ug.

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  IP experiment of HepG2 using 15681-1-AP

  IP result of anti-KHK (IP:15681-1-AP, 4ug; Detection:15681-1-AP 1:1000) with HepG2 cells lysate 960 ug.

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  IHC staining of human liver using 15681-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15681-1-AP (KHK Antibody) at dilution of 1:400 (under 10x lens).

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  IHC staining of human liver using 15681-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15681-1-AP (KHK Antibody) at dilution of 1:400 (under 40x lens).

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  IHC staining of human liver using 15681-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15681-1-AP (KHK Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 15681-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15681-1-AP (KHK Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HepG2 using 15681-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 15681-1-AP (KHK antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 15681-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using KHK antibody (15681-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse liver tissue, human liver tissue, rat liver tissue
• Positive IP detected in: mouse liver tissue, HepG2 cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 1 publications below

Product Information

15681-1-AP targets KHK in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KHK fusion protein Ag8182
• Full Name: ketohexokinase (fructokinase)
• Calculated Molecular Weight: 298 aa, 33 kDa
• Observed Molecular Weight: 33-35 kDa
• GenBank Accession Number: BC006233
• Gene Symbol: KHK
• Gene ID (NCBI): 3795
• RRID: AB_2130496
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ketohexokinase catalyzes conversion of fructose to fructose-1-phosphate, it is the first enzyme with a specialized pathway that catabolizes dietary fructose. Fructose metabolism seems to provide cancer cells with the supplementary fuel required for proliferation and metastasis.

Publications

Species	Application	Title
mouse	WB,IHC	Cell Metab; High dietary fructose promotes hepatocellular carcinoma progression by enhancing O-GlcNAcylation via microbiota-derived acetate; Authors - Peng Zhou
human	WB	Adv Sci (Weinh); NAT10/ac4C/FOXP1 Promotes Malignant Progression and Facilitates Immunosuppression by Reprogramming Glycolytic Metabolism in Cervical Cancer; Authors - Xiaona Chen
human	WB	Brain; C9orf72 expansion within astrocytes reduces metabolic flexibility in amyotrophic lateral sclerosis.; Authors - Scott P Allen
human	WB	Cancer Sci; Identification of TPI1 As a potential therapeutic target in pancreatic cancer with dependency of TP53 mutation using multi-omics analysis; Authors - Tomoaki Toyoda
mouse	WB	Endocrinology; Hepatic but not intestinal FBP1 is required for fructose metabolism and tolerance; Authors - Qiufang Bai
human	WB	Biochem Biophys Res Commun; Ketohexokinase is involved in fructose utilization and promotes tumor progression in glioma.; Authors - Wenbo Gao