KHDRBS3 Polyclonal antibody

• Featured Product
• KD/KO Validated

KHDRBS3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Reviews (3)

Cat no : 13563-1-AP

Synonyms

Etle, etoile, KHDRBS3, RNA binding protein T Star, SALP, Sam68 like mammalian protein 2, SLM 2, SLM2, T STAR, TSTAR

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Validation Data Gallery

• 

  WB analysis of mouse brain using 13563-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13563-1-AP (KHDRBS3 Antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 13563-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13563-1-AP (KHDRBS3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 13563-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 13563-1-AP (KHDRBS3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BGC-823 using 13563-1-AP

  BGC-823 cells were subjected to SDS PAGE followed by western blot with 13563-1-AP (KHDRBS3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 13563-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13563-1-AP (KHDRBS3 Antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human gliomas using 13563-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 13563-1-AP (KHDRBS3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human gliomas using 13563-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 13563-1-AP (KHDRBS3 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse brain tissue, BGC-823 cells, A549 cells, human brain tissue
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Product Information

13563-1-AP targets KHDRBS3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KHDRBS3 fusion protein Ag4438
• Full Name: KH domain containing, RNA binding, signal transduction associated 3
• Calculated Molecular Weight: 346 aa, 38, 8 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC032606
• Gene Symbol: KHDRBS3
• Gene ID (NCBI): 10656
• RRID: AB_2130319
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KH domain-containing, RNA-binding, signal transduction-associated protein 3 (KHDRBS3, SLM2) is a RNA-binding protein that plays a role in the regulation of alternative splicing and influences mRNA splice site selection and exon inclusion (15901763). May play a role as a negative regulator of cell growth. Inhibits cell proliferation. Involved in splice site selection of vascular endothelial growth factor. Induces an increased concentration-dependent incorporation of exon in CD44 pre-mRNA by direct binding to purine-rich exonic enhancer. RNA-binding abilities are down-regulated by tyrosine kinase PTK6. Involved in post-transcriptional regulation of HIV-1 gene expression (11741900).Ubiquitous with higher expression in testis, skeletal muscle and brain. Expressed in the kidney only in podocytes, the glomerular epithelial cells of the kidney. Strongly expressed after meiosis.This antibody is a rabbit polyclonal antibody raised against a full-length human KHDRBS3 protein.The observed molecular weight of KHDRBS is about 55kd.

Publications

Species	Application	Title
human	IHC	Acta Neuropathol; Transportin 1 accumulates specifically with FET proteins but no other transportin cargos in FTLD-FUS and is absent in FUS inclusions in ALS with FUS mutations.; Authors - Neumann Manuela M
mouse	WB	PLoS Genet; The tissue-specific RNA binding protein T-STAR controls regional splicing patterns of neurexin pre-mRNAs in the brain.; Authors - Ehrmann Ingrid I
human	WB	Am J Reprod Immunol; Long non-coding RNA TPT1-AS1 inhibits ferroptosis in ovarian cancer by regulating GPX4 via CREB1 regulation; Authors - Lei Cao
human	WB	Cell Death Dis; KHDRBS3 regulates the permeability of blood-tumor barrier via cDENND4C/miR-577 axis.; Authors - Peiqi Wu ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Aleksandr (Verified Customer) (11-10-2024)

Antibodies were used to detect Khdrbs3 in human RKO cells

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: RKO

KHDRBS3 Antibody Western Blot validation (1:1000 dilution) in RKO (Cat no:13563-1-AP)

FH

Tsimafei (Verified Customer) (08-07-2024)

Great antibody

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mESCs

KHDRBS3 Antibody Western Blot validation (1:2000 dilution) in mESCs (Cat no:13563-1-AP)

FH

Julia (Verified Customer) (08-07-2024)

Antibody used for 30ug of mESC protein extract. There are few bands and strong signal at ~55kDa so it can be diluted even more than 1:10000 for similar set-up.

• Applications: Western Blot
• Primary Antibody Dilution: 1:10000
• Cell Tissue Type: mESC

KHDRBS3 Antibody Western Blot validation (1:10000 dilution) in mESC (Cat no:13563-1-AP)