KGA/GAC Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

KGA/GAC Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3A12A1

Cat no : 66265-1-PBS

Synonyms

GLS, KGA, GAM, GAC, EC:3.5.1.2

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Validation Data Gallery

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  WB analysis of NIH/3T3 using 66265-1-Ig (same clone as 66265-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66265-1-Ig (GLS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66265-1-PBS in a different storage buffer formulation.

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  WB analysis of rat liver using 66265-1-Ig (same clone as 66265-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 66265-1-Ig (GLS Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66265-1-PBS in a different storage buffer formulation.

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  WB analysis of fetal human brain using 66265-1-Ig (same clone as 66265-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66265-1-Ig (GLS Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66265-1-PBS in a different storage buffer formulation.

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  WB analysis of U-251 using 66265-1-Ig (same clone as 66265-1-PBS)

  U-251 cells were subjected to SDS PAGE followed by western blot with 66265-1-Ig (GLS Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66265-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66265-1-Ig (same clone as 66265-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66265-1-Ig (GLS Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66265-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 66265-1-Ig (same clone as 66265-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66265-1-Ig (GLS antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66265-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 66265-1-Ig (same clone as 66265-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66265-1-Ig (GLS antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66265-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 66265-1-Ig (same clone as 66265-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66265-1-Ig (GLS antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66265-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 66265-1-Ig (same clone as 66265-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66265-1-Ig (GLS antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66265-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 66265-1-Ig (same clone as 66265-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 66265-1-Ig (GLS antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66265-1-PBS in a different storage buffer formulation.

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  IF Staining of L02 using 66265-1-Ig (same clone as 66265-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed L02 cells using KGA/GAC antibody (66265-1-Ig, Clone: 3A12A1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66265-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66265-1-Ig (same clone as 66265-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed HepG2 cells using 66265-1-Ig(GLS antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66265-1-PBS in a different storage buffer formulation.

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Product Information

66265-1-PBS targets KGA/GAC in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: KGA/GAC fusion protein Ag24227
• Full Name: glutaminase
• Calculated Molecular Weight: 669 aa, 73 kDa
• Observed Molecular Weight: 58 kDa, 65 kDa
• GenBank Accession Number: BC038507
• Gene Symbol: GLS
• Gene ID (NCBI): 2744
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

GLS, also named as GLS1 and KIAA0838, belongs to the glutaminase family. It catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Glutaminase-, glutamate-, and taurine-immunoreactive neurons develop neurofibrillary tangles in Alzheimer's disease(PMID:1672808).The glutaminase band in AA/C1 cells is more intense than in HT29 cells, in accordance with measurements of glutaminase activity, and had the same molecular mass of approx. 63 kDa. The bands for both cell lines are clearly different in size from both rat liver glutaminase (58 kDa) and rat kidney glutaminase (65 kDa)(PMID:12408749). It also reveals a molecular weight of 83-84 kDa as a phosphate-dependent glutaminase(PMID:447624;7512428). It has 3 isoforms named as KGA, GAM, GAC. This antibody can recognize KGA and GAC.