KEPI Polyclonal antibody

• Featured Product
• KD/KO Validated

KEPI Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 24933-1-AP

Synonyms

CPI17 like, KEPI, Kinase enhanced PP1 inhibitor, NY BR 81, PPP1R14C

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Validation Data Gallery

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  WB analysis using 24933-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24933-1-AP (KEPI antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 24933-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24933-1-AP (KEPI antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 24933-1-AP

  WB result of KEPI antibody (24933-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KEPI transfected HeLa cells.

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, MDA-MB-231 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

24933-1-AP targets KEPI in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KEPI fusion protein Ag20645
• Full Name: protein phosphatase 1, regulatory (inhibitor) subunit 14C
• Calculated Molecular Weight: 18 kDa
• Observed Molecular Weight: 20-22 kDa
• GenBank Accession Number: BC015773
• Gene Symbol: PPP1R14C
• Gene ID (NCBI): 81706
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PPP1R14C, found as a morphine‐regulated brain gene, encodes protein phosphatase 1 regulatory inhibitor subunit 14C, and is identified as a potent inhibitor of PP1. Horvath and colleagues reported that PPP1R14C inhibited the release of neurotransmitters and promoted neuronal exocytosis by retaining synaptosome associated protein 25 (SNAP25) at a phosphorylated state. Moreover, overexpression of PPP1R14C increased the phosphorylation of RB transcriptional co‐repressor 1 (RB1) to protect leukemic cells from chemotherapy.