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KEAP1 Polyclonal antibody, PBS Only

KEAP1 Polyclonal Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

Cat no : 30041-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KEAP1 Polyclonal antibody (30041-1-AP)

    WB analysis using 30041-1-AP (same clone as 30041-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30041-1-AP (KEAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30041-1-PBS in a different storage buffer formulation.

Product Information

30041-1-PBS targets KEAP1 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KEAP1 fusion protein Ag32512
Full Name kelch-like ECH-associated protein 1
Calculated Molecular Weight 624 aa, 70 kDa
Observed Molecular Weight55-70 kDa
GenBank Accession NumberBC002930
Gene Symbol KEAP1
Gene ID (NCBI) 9817
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

KEAP1, also named as INRF2, KIAA0132 and KLHL19, is part of a multiprotein complex that contains the CUL3-ROC1 ubiquitin ligase, which can ubiquitinate the N-terminal domain of NRF2[PMID: 20173742]. Two molecules of KEAP1 bind to two distinct sites in the N-terminal region of NRF2, the ETGE and DLG sites, which affect the KEAP1-NRF2 interaction and/or its physiological consequences[PMID: 22215675]. KEAP1 retains NFE2L2/NRF2 in the cytosol. It functions as substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1[PMID: 20427290]. It also retains BPTF in the cytosol. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human KEAP1.