VEGFR2/KDR Monoclonal Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Mouse / IgG1

Detection: Mouse / IgG1

Reactivity

Capture: human

Detection: human

Applications

Sandwich ELISA

Range

Sandwich ELISA: 78.1-5000 pg/mL

Cat No : MP50080-1

Print datasheet

Synonyms

CD309, Fetal liver kinase 1, FLK 1, FLK1, KDR, Kinase insert domain receptor, VEGFR, VEGFR 2, VEGFR2

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Validation Data Gallery

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  Sandwich ELISA standard curve of MP50080-1

  Sandwich ELISA standard curve of MP50080-1, KDR Monoclonal Matched Antibody Pair - PBS only. 68689-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0218. 68689-2-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

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Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP50080-1 targets VEGFR2/KDR in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 78.1-5000 pg/mL (Sandwich ELISA);

Capture antibody: VEGFR2/KDR Monoclonal antibody, PBS Only (Capture) 68689-1-PBS (3A3F5). 100 μg. Concentration 1 mgl/ml.

Detection antibody: VEGFR2/KDR Monoclonal antibody, PBS Only (Detector) 68689-2-PBS (3C10A1). 100 μg. Concentration 1 mgl/ml.

Alternative VEGFR2/KDR matched antibody pairs: MP00394-1, MP00394-2, MP00394-3

Matched pair protein standard: Eg0218

Unconjugated mouse monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

• Capture antibody
• Detection antibody

• Catalog Number: 68689-1-PBS
• Tested Reactivity: human
• Immunogen: Recombinant protein
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Affinity:
  K_D=6.32 x 10^-12M
  K_Off=3.94 x 10^-6M
  K_On=6.24 x 10^5M
• Full Name: kinase insert domain receptor (a type III receptor tyrosine kinase)
• Synonyms: KDR, VEGFR2, Protein-tyrosine kinase receptor flk-1, FLK-1, EC:2.7.10.1
• GenBank accession number: NM_002253
• Gene symbol: KDR
• Uniprot: P35968-1
• Gene ID (NCBI): 3791
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

• Catalog Number: 68689-2-PBS
• Tested Reactivity: human
• Immunogen: Recombinant protein
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Affinity:
  K_D=3.25 x 10^-10M
  K_Off=2.90 x 10^-4M
  K_On=8.91 x 10^5M
• Full Name: kinase insert domain receptor (a type III receptor tyrosine kinase)
• Synonyms: KDR, VEGFR2, Protein-tyrosine kinase receptor flk-1, FLK-1, EC:2.7.10.1
• GenBank accession number: NM_002253
• Gene symbol: KDR
• Uniprot: P35968-1
• Gene ID (NCBI): 3791
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.