KDM3A/JMJD1A Polyclonal antibody

• Featured Product
• KD/KO Validated

KDM3A/JMJD1A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Publications

43

Cat no : 12835-1-AP

Synonyms

KDM3A, KDM3A,JMJD1A, JMJD1, JmjC domain-containing histone demethylation protein 2A, JHMD2A

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Validation Data Gallery

• 

  WB analysis of HeLa using 12835-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12835-1-AP (KDM3A,JMJD1A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 12835-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12835-1-AP (KDM3A,JMJD1A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 12835-1-AP

  IP result of anti-KDM3A,JMJD1A (IP:12835-1-AP, 5ug; Detection:12835-1-AP 1:1000) with HeLa cells lysate 2500ug.

• 

  IHC staining of human ovary tumor using 12835-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 12835-1-AP (KDM3A,JMJD1A antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 12835-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 12835-1-AP (KDM3A,JMJD1A antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 12835-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 12835-1-AP (KDM3A,JMJD1A antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 12835-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 12835-1-AP (KDM3A,JMJD1A antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 12835-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 12835-1-AP (KDM3A,JMJD1A antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 12835-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 12835-1-AP (KDM3A,JMJD1A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 12835-1-AP

  Immunofluorescent analysis of HepG2 cells, using KDM3A antibody 12835-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human ovary tumor tissue, human kidney tissue, human ovary tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 16 publications below
• WB: See 36 publications below
• IHC: See 11 publications below
• IF: See 5 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below
• ChIP: See 6 publications below

Product Information

12835-1-AP targets KDM3A/JMJD1A in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KDM3A/JMJD1A fusion protein Ag3581
• Full Name: lysine (K)-specific demethylase 3A
• Calculated Molecular Weight: 150 kDa
• Observed Molecular Weight: 150 kDa
• GenBank Accession Number: BC038297
• Gene Symbol: KDM3A/JMJD1A
• Gene ID (NCBI): 55818
• RRID: AB_1072935
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KDM3A, also name as JMJD1A, JHDM2A, JMJD1, JMJD1A, KIAA0742 and TSGA, belongs to the JHDM2 histone demethylase family. It is a histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. It is involved in spermatogenesis by regulating expression of target genes such as PRM1 and TMP1 which are required for packaging and condensation of sperm chromatin. It is involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1. (PubMed:16603237), JMJD1A is a useful biomarker for hypoxic tumor cells and a prognostic marker that could be a promising therapeutic target against CRC. (PMID:20823141) This antibody recognize the N-term of KDM3A(JMJD1A).

Publications

Species	Application	Title
human	WB	Adv Sci (Weinh); KDM3A Ablation Activates Endogenous Retrovirus Expression to Stimulate Antitumor Immunity in Gastric Cancer; Authors - Jiabin Zheng ; KD Validated
human	WB,IHC,IF	Nat Commun; Normal stroma suppresses cancer cell proliferation via mechanosensitive regulation of JMJD1a-mediated transcription.; Authors - Riina Kaukonen ; KD Validated
human	WB	Nat Commun; Delaying histone deacetylase response to injury accelerates conversion into repair Schwann cells and nerve regeneration.; Authors - Valérie Brügger
human	WB	Biomaterials; Lysine demethylase KDM3A regulates nanophotonic hyperthermia resistance generated by 2D silicene in breast cancer.; Authors - Chao He
mouse	WB	EMBO J; Coordinated removal of repressive epigenetic modifications during induced reversal of cell identity.; Authors - Khoa A Tran
human	WB	Proc Natl Acad Sci U S A; Histone demethylase JMJD1A promotes alternative splicing of AR variant 7 (AR-V7) in prostate cancer cells.; Authors - Lingling Fan ; KD Validated

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