KDM2A Polyclonal antibody

KDM2A Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, IHC, CoIP, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 24311-1-AP

Synonyms

CXXC8, F box protein FBL7, F box protein Lilina, F box/LRR repeat protein 11, FBL11, FBL7, FBXL11, JHDM1A, KDM2A, KIAA1004, LILINA, lysine (K) demethylase 2A, Lysine specific demethylase 2A

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Validation Data Gallery

• 

  WB analysis of Jurkat using 24311-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 24311-1-AP (KDM2A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 24311-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 24311-1-AP (KDM2A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 24311-1-AP

  IP result of anti-KDM2A (IP:24311-1-AP, 4ug; Detection:24311-1-AP 1:600) with HeLa cells lysate 1600ug.

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Tested Applications

• Positive WB detected in: Jurkat cells, NIH/3T3 cells
• Positive IP detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

The immunogen of 24311-1-AP is KDM2A Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KDM2A fusion protein Ag19448
• Full Name: lysine (K)-specific demethylase 2A
• Calculated Molecular Weight: 1162 aa, 133 kDa
• Observed Molecular Weight: 126 kDa
• GenBank Accession Number: BC064360
• Gene Symbol: KDM2A
• Gene ID (NCBI): 22992
• RRID: AB_2879488
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KDM2A(Lysine-specific demethylase 2A) is also named as CXXC8, FBL7, FBXL11, JHDM1A, KIAA1004 and belongs to the JHDM1 histone demethylase family. It is required to maintain the heterochromatic state, and playing a role in epigenetic silencing. KDM2A is one of the four subunits of the E3 ubiquitin protein ligase complex (SCF) with SKP1, CUL1, RBX1, Fbox protein bringing ubiquitin conjugating enzymes to substrates recruited by F-box.

Publications

Species	Application	Title
human	WB,IF,CoIP	Cell Oncol (Dordr); Hepatitis B virus core protein stabilizes RANGAP1 to upregulate KDM2A and facilitate hepatocarcinogenesis; Authors - Hong-Juan You
human	WB	J Cell Mol Med; Circular RNA circFOXO3 regulates KDM2A by targeting miR-214 to promote tumor growth and metastasis in oral squamous cell carcinoma.; Authors - Yilong Ai
human	WB	J Cell Physiol; The Ubiquitin Ligase SCF(FBXW7α) Promotes ATA3 Degradation.; Authors - Nan Song
human	IP, WB	Cell Rep; Enhancer RNAs Mediate Estrogen-Induced Decommissioning of Selective Enhancers by Recruiting ERα and Its Cofactor.; Authors - Mei Yang
human	WB,IHC	Cell Death Dis; CircRNF144B/miR-342-3p/FBXL11 axis reduced autophagy and promoted the progression of ovarian cancer by increasing the ubiquitination of Beclin-1; Authors - Wenting Song