KDELC2 Polyclonal antibody

KDELC2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 23345-1-AP

Synonyms

Protein O-xylosyltransferase POGLUT3, Protein O-glucosyltransferase 3, POGLUT3, KDEL motif-containing protein 2, EC:2.4.2.-

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Validation Data Gallery

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  WB analysis of HeLa using 23345-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23345-1-AP (KDELC2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 23345-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23345-1-AP (KDELC2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SMMC-7721 using 23345-1-AP

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 23345-1-AP (KDELC2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 23345-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 23345-1-AP (KDELC2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 23345-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 23345-1-AP (KDELC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 23345-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 23345-1-AP (KDELC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 23345-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 23345-1-AP (KDELC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 23345-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 23345-1-AP (KDELC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 23345-1-AP

  Immunofluorescent analysis of HepG2 cells using 23345-1-AP (KDELC2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, human placenta tissue, SMMC-7721 cells
• Positive IHC detected in: human breast cancer tissue, human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

23345-1-AP targets KDELC2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KDELC2 fusion protein Ag19929
• Full Name: KDEL (Lys-Asp-Glu-Leu) containing 2
• Calculated Molecular Weight: 507 aa, 59 kDa
• Observed Molecular Weight: 62 kDa
• GenBank Accession Number: BC036526
• Gene Symbol: KDELC2
• Gene ID (NCBI): 143888
• RRID: AB_2879260
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.