KChIP1 Monoclonal antibody, PBS Only

KChIP1 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3D6C1

Cat no : 66439-1-PBS

Synonyms

KCHIP1, KCNIP1, VABP, Vesicle APC binding protein

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Validation Data Gallery

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  WB analysis of human cerebellum using 66439-1-Ig (same clone as 66439-1-PBS)

  human cerebellum tissue were subjected to SDS PAGE followed by western blot with 66439-1-Ig (KChIP1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66439-1-PBS in a different storage buffer formulation.

• 

  WB analysis of fetal human brain using 66439-1-Ig (same clone as 66439-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66439-1-Ig (KChIP1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66439-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human spleen using 66439-1-Ig (same clone as 66439-1-PBS)

  human spleen tissue were subjected to SDS PAGE followed by western blot with 66439-1-Ig (KChIP1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66439-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 66439-1-Ig (same clone as 66439-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66439-1-Ig (KChIP1 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66439-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 66439-1-Ig (same clone as 66439-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66439-1-Ig (KChIP1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66439-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 66439-1-Ig (same clone as 66439-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66439-1-Ig (KChIP1 antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66439-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 66439-1-Ig (same clone as 66439-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66439-1-Ig (KChIP1 antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66439-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 66439-1-Ig (same clone as 66439-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using KChIP1 antibody (66439-1-Ig, Clone: 3D6C1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66439-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 66439-1-Ig (same clone as 66439-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using KChIP1 antibody (66439-1-Ig, Clone: 3D6C1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66439-1-PBS in a different storage buffer formulation.

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Product Information

66439-1-PBS targets KChIP1 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: KChIP1 fusion protein Ag5494
• Full Name: Kv channel interacting protein 1
• Calculated Molecular Weight: 227 aa, 27 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC050375
• Gene Symbol: KCNIP1
• Gene ID (NCBI): 30820
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Human K(v) channel interacting protein 1 (KCHIP1) is a new member of the neural calcium binding protein superfamily. Members of the KCNIP family are small calcium binding proteins. They all have EF-hand-like domains, and differ from each other in the N-terminus. They are integral subunit components of native Kv4 channel complexes. They may regulate A-type currents, and hence neuronal excitability, in response to changes in intracellular calcium. KChIP1 is a neuronal calcium sensor protein that is predominantly expressed at GABAergic synapses and it has been related with modulation of K(+) channels, GABAergic transmission and cell death.