KCTD10 Polyclonal antibody

• Featured Product
• KD/KO Validated

KCTD10 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 27279-1-AP

Synonyms

ULR061, Potassium channel tetramerization domain-containing protein 10, MSTP028, hBACURD3, BTB/POZ domain-containing protein KCTD10

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Validation Data Gallery

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  WB analysis of Jurkat using 27279-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 27279-1-AP (KCTD10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U2OS using 27279-1-AP

  U2OS cells were subjected to SDS PAGE followed by western blot with 27279-1-AP (KCTD10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 27279-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 27279-1-AP (KCTD10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 27279-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 27279-1-AP (KCTD10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 27279-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 27279-1-AP (KCTD10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 27279-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 27279-1-AP (KCTD10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 27279-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 27279-1-AP (KCTD10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 27279-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 27279-1-AP (KCTD10 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 27279-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 27279-1-AP (KCTD10 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Jurkat cells, HEK-293 cells, HeLa cells, K-562 cells, MCF-7 cells, U2OS cells
• Positive IHC detected in: human intrahepatic cholangiocarcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 4 publications below

Product Information

27279-1-AP targets KCTD10 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KCTD10 fusion protein Ag25971
• Full Name: potassium channel tetramerisation domain containing 10
• Calculated Molecular Weight: 313 aa, 35 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC040062
• Gene Symbol: KCTD10
• Gene ID (NCBI): 83892
• RRID: AB_2880827
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KCTD10 is a member of the polymerase delta-interacting protein 1 (PDIP1) gene family, which consists of 3 members, PDIP1, KCTD10 and TNFAIP1. KCTD10 is inducible by TNF-α, interacts with PCNA and the small subunit (p50) of DNA polymerase δ. Previous reports have shown that KCTD10 is essential for mammalian development. Further functional studies showed that KCTD10 interacts with proliferating cell nuclear antigen (PCNA) and plays an important role in DNA synthesis.

Publications

Species	Application	Title
human	WB	Mol Cell; Unbiased interrogation of functional lysine residues in human proteome; Authors - Ying Bao
human	WB	Nat Commun; Regulation of TRIF-mediated innate immune response by K27-linked polyubiquitination and deubiquitination.; Authors - Xin Wu ; KO Validated
mouse	WB	Front Cell Dev Biol; Cullin3-TNFAIP1 E3 Ligase Controls Inflammatory Response in Hepatocellular Carcinoma Cells via Ubiquitination of RhoB.; Authors - Yue Liu ; KD Validated
human,rat	WB	Genet Res (Camb); Inhibition of KCTD10 Affects Diabetic Retinopathy Progression by Reducing VEGF and Affecting Angiogenesis; Authors - Yun Feng ; KD Validated