mouse lung tissue were subjected to SDS PAGE followed by western blot with 14135-1-AP (Kir2.2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
mouse lung tissue were subjected to SDS PAGE followed by western blot with 14135-1-AP (Kir2.2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of rat lung using 14135-1-AP
rat lung tissue were subjected to SDS PAGE followed by western blot with 14135-1-AP (Kir2.2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
rat lung tissue were subjected to SDS PAGE followed by western blot with 14135-1-AP (Kir2.2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
FC experiment of HEK-293 using 14135-1-AP
1x10^6 HEK-293 cells were intracellularly stained with 0.5 ug Kir2.2 Polyclonal antibody (14135-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.5 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
1x10^6 HEK-293 cells were intracellularly stained with 0.5 ug Kir2.2 Polyclonal antibody (14135-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.5 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
mouse lung tissue were subjected to SDS PAGE followed by western blot with 14135-1-AP (Kir2.2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of rat lung using 14135-1-AP
rat lung tissue were subjected to SDS PAGE followed by western blot with 14135-1-AP (Kir2.2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
FC (INTRA) Figures
FC experiment of HEK-293 using 14135-1-AP
1x10^6 HEK-293 cells were intracellularly stained with 0.5 ug Kir2.2 Polyclonal antibody (14135-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.5 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
Kir2.2 Polyclonal antibody
Catalog Number
14135-1-AP
Citations
-
Dilutions
WB : 1:500-1:2400 FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
Applications
WB, FC (Intra), ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
