KCND3 Recombinant antibody

KCND3 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240578F12

Cat no : 83640-5-RR

Synonyms

KV4.3, KSHIVB, KCND3S, KCND3L, 240578F12

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Validation Data Gallery

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  WB analysis of rat brain using 83640-5-RR

  rat brain tissue were subjected to SDS PAGE followed by western blot with 83640-5-RR (KCND3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83640-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83640-5-RR against Human KCND3 were performed. The affinity constant is 0.712 nM.

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Tested Applications

• Positive WB detected in: rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83640-5-RR is KCND3 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: KCND3 fusion protein Ag22183
• Full Name: potassium voltage-gated channel, Shal-related subfamily, member 3
• Calculated Molecular Weight: 655 aa, 73 kDa
• Observed Molecular Weight: 73 kDa
• GenBank Accession Number: BC113477
• Gene Symbol: KCND3
• Gene ID (NCBI): 3752
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KCND3 gene encodes the voltage-gated potassium ion channel subfamily D member 3, a six trans-membrane protein (Kv4.3), involved in the transient outward K+ current. KCND3 gene defect causes both cardiological and neurological syndromes. Kv4.3 contributes to the cardiac transient outward potassium current (Ito1), the main contributing current to the repolarizing phase 1 of the cardiac action potential.