KCND3 Polyclonal antibody

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  WB analysis using 25468-1-AP

  Rat brain tissues (heated at 100 ° C, heated at 37℃) lysates were subjected to SDS PAGE followed by western blot with 25468-1-AP (KCND3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of rat brain using 25468-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 25468-1-AP (KCND3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 25468-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 25468-1-AP (KCND3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 25468-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 25468-1-AP (KCND3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 25468-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 25468-1-AP (KCND3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 25468-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25468-1-AP (KCND3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 25468-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25468-1-AP (KCND3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

KCND3 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 25468-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

KV4.3, KSHIVB, KCND3S, KCND3L

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Tested Applications

• Positive WB detected in: rat brain tissue, 37℃ incubated rat brain tissue
• Positive IHC detected in: mouse brain tissue, mouse cerebellum tissue, rat brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25468-1-AP targets KCND3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KCND3 fusion protein Ag22183
• Full Name: potassium voltage-gated channel, Shal-related subfamily, member 3
• Calculated Molecular Weight: 655 aa, 73 kDa
• Observed Molecular Weight: 73 kDa
• GenBank Accession Number: BC113477
• Gene Symbol: KCND3
• Gene ID (NCBI): 3752
• RRID: AB_3669478
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UK17
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KCND3 gene encodes the voltage-gated potassium ion channel subfamily D member 3, a six trans-membrane protein (Kv4.3), involved in the transient outward K+ current. KCND3 gene defect causes both cardiological and neurological syndromes. Kv4.3 contributes to the cardiac transient outward potassium current (Ito1), the main contributing current to the repolarizing phase 1 of the cardiac action potential.