KCNA3 Polyclonal antibody

• Featured Product
• KD/KO Validated

KCNA3 Polyclonal Antibody for WB, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF-P, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 14079-1-AP

Synonyms

Kv1.3, MK3, HUKIII, HPCN3, HLK3

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Validation Data Gallery

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  WB analysis of A549 using 14079-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 14079-1-AP (Kv1.3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 14079-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 14079-1-AP (Kv1.3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 14079-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14079-1-AP (Kv1.3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of Rat brain using 14079-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Rat brain tissue using Kv1.3 antibody (14079-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells, mouse thymus tissue
• Positive IF-P detected in: Rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 1 publications below

Product Information

14079-1-AP targets KCNA3 in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KCNA3 fusion protein Ag5204
• Full Name: potassium voltage-gated channel, shaker-related subfamily, member 3
• Calculated Molecular Weight: 575 aa, 64 kDa
• Observed Molecular Weight: 52 kDa, 75 kDa
• GenBank Accession Number: BC035059
• Gene Symbol: Kv1.3
• Gene ID (NCBI): 3738
• RRID: AB_2878011
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Kv1.3 ( also known as KCNA3, MK3, HGK5, HLK3, PCN3, HPCN3) contains six membrane-spanning domains with a shaker-type repeat in the fourth segment. It belongs to the delayed rectifier class, members of which allow nerve cells to efficiently repolarize following an action potential. It plays an essential role in T cell proliferation and activation. Kv1.3 channels are expressed in in various tissues and cell types including brain, vascular smooth muscle cells and leucocytes. Malfunction of Kv1.3 can alter the immune response, elicit neurotoxic effects or impact on cancer growth(PMID: 24305723; 24133455). 14079-1-AP can detect two isoforms of Kv1.3 other than the canonical channel (60 KDa) : A longer isoform (70 KDa) and a truncated isoform (43 KDa).

Publications

Species	Application	Title
mouse	WB	Cell Mol Immunol; NLRP3-dependent pyroptosis is required for HIV-1 gp120-induced neuropathology.; Authors - Xiaolong He ; KD Validated
mouse	WB,IF	Int Immunopharmacol; Clarithromycin attenuates airway epithelial-mesenchymal transition in ovalbumin-induced asthmatic mice through modulation of Kv1.3 channels and PI3K/Akt signaling; Authors - Bingqing Sun
human	WB	FASEB J; The Kv1.3 ion channel acts as a host factor restricting viral entry.; Authors - Yange Lang
rat	WB	Int J Clin Exp Med; Inhibition of 15-lipoxygenase (15-LOX) reverses hypoxia-induced down-regulation of potassium channels Kv1.5 and Kv2.1Inhibition of 15-lipoxygenase (15-LOX) reverses hypoxia-induced down-regulation of potassium channels Kv1.5 and Kv2.1.; Authors - Wenjuan Liu