KBTBD5 Polyclonal antibody

KBTBD5 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21836-1-AP

Synonyms

KBTBD5, KLHL40, Sarcosynapsin, SRYP

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Validation Data Gallery

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  WB analysis of mouse skeletal muscle using 21836-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21836-1-AP (KBTBD5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse skeletal muscle using 21836-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 21836-1-AP (KBTBD5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 21836-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 21836-1-AP (KBTBD5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skeletal muscle using 21836-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 21836-1-AP (KBTBD5 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 21836-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 21836-1-AP (KBTBD5 antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue
• Positive IHC detected in: mouse skeletal muscle tissue, human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21836-1-AP targets KBTBD5 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KBTBD5 fusion protein Ag16430
• Full Name: kelch repeat and BTB (POZ) domain containing 5
• Calculated Molecular Weight: 621 aa, 69 kDa
• Observed Molecular Weight: 69 kDa
• GenBank Accession Number: BC110491
• Gene Symbol: KBTBD5
• Gene ID (NCBI): 131377
• RRID: AB_2878923
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KBTBD5, also known as KLHL40 and SRYP, contains a BACK domain, a BTB/POZ domain and 5 Kelch repeats. It is expressed predominantly in skeletal muscle. The KLHL40 gene mutation is responsible for a severe and potentially fatal neuromyopathy (PMID: 38397198).