KBTBD2 Polyclonal antibody

KBTBD2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 13871-1-AP

Synonyms

BKLHD1, KBTBD2, KIAA1489

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Validation Data Gallery

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  WB analysis of COLO 320 using 13871-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 13871-1-AP (KBTBD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13871-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13871-1-AP (KBTBD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: COLO 320 cells, A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13871-1-AP targets KBTBD2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KBTBD2 fusion protein Ag4823
• Full Name: kelch repeat and BTB (POZ) domain containing 2
• Calculated Molecular Weight: 622 aa, 71 kDa
• Observed Molecular Weight: 60-70 kDa
• GenBank Accession Number: BC047107
• Gene Symbol: KBTBD2
• Gene ID (NCBI): 25948
• RRID: AB_2296330
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Kelch repeat and BTB domain containing 2(KBTBD2), also known as BTB and kelch domain-containing protein 1, encodes a BTB-Kelch family subunit that p85α-specific recognition for the Cullin3-based E3 ubiqitin ligase complex, which regulates p85α protein ubiquitination and subsequently regulates phosphoinositide-3 kinases (PI3K) signaling (PMID:27708159). However, the expression profile and functional significance of Kbtbd2 is still largely unknown. The Human Protein Atlas which is an online source shows high Kbtbd2 expression in the spleen and thymus, suggesting that Kbtbd2 might involved in immunization (PMID:29331106). In addition, Kbtbd2 is related to Src-induced phosphorylation of protein, spermatogonial stem cell activity(PMID:15623525, 29331106). A 60-70 kDa band has also been reported(PMID:27708159).