KATNB1 Polyclonal antibody

• Featured Product
• KD/KO Validated

KATNB1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

11

Cat no : 14969-1-AP

Synonyms

p80 katanin, Katanin p80 WD40 repeat-containing subunit B1, Katanin p80 subunit B1, KAT

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Validation Data Gallery

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  WB analysis using 14969-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 14969-1-AP (KATNB1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 14969-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14969-1-AP (KATNB1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 14969-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14969-1-AP (KATNB1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 14969-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 14969-1-AP (KATNB1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 14969-1-AP

  IP result of anti-KATNB1 (IP:14969-1-AP, 3ug; Detection:14969-1-AP 1:500) with HeLa cells lysate 3000ug.

• 

  IF Staining of HeLa using 14969-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 14969-1-AP (KATNB1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A431 cells, mouse skeletal muscle tissue, HepG2 cells
• Positive IP detected in: HeLa cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IF: See 6 publications below

Product Information

14969-1-AP targets KATNB1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KATNB1 fusion protein Ag6836
• Full Name: katanin p80 (WD repeat containing) subunit B 1
• Calculated Molecular Weight: 72 kDa
• Observed Molecular Weight: 72-80 kDa
• GenBank Accession Number: BC001353
• Gene Symbol: KATNB1
• Gene ID (NCBI): 10300
• RRID: AB_10637861
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Katanin p80 WD40-containing subunit B1 is a protein that in humans is encoded by the KATNB1 gene. Microtubules, polymers of alpha and beta tubulin subunits, form the mitotic spindle of a dividing cell and help to organize membranous organelles during interphase. Katanin is a heterodimer that consists of a 60 kDa ATPase (p60 subunit A 1) and an 80 kDa accessory protein (p80 subunit B 1). The p60 subunit acts to sever and disassemble microtubules, while the p80 subunit targets the enzyme to the centrosome. Katanin is a member of the AAA family of ATPases.

Publications

Species	Application	Title
mouse	WB, IF	Neuron; Katanin p80 regulates human cortical development by limiting centriole and cilia number.; Authors - Wen F Hu
human	WB	Dev Cell; Microtubule Minus-End Stabilization by Polymerization-Driven CAMSAP Deposition.; Authors - Kai Jiang
human	IF, WB	J Cell Biol; WDR62 localizes katanin at spindle poles to ensure synchronous chromosome segregation.; Authors - Amanda Guerreiro
human	WB, IF	J Cell Biol; WDR62 regulates spindle dynamics as an adaptor protein between TPX2/Aurora A and katanin.; Authors - Junjie Huang
mouse	IF	J Neurosci; Functional inhibition of katanin affects synaptic plasticity; Authors - Franco L Lombino
human	WB	Structure; Structural Basis of Formation of the Microtubule Minus-End-Regulating CAMSAP-Katanin Complex.; Authors - Kai Jiang ; KO Validated