PCAF Polyclonal antibody

PCAF Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 28770-1-AP

Synonyms

CAF, Histone acetylase PCAF, Histone acetyltransferase PCAF, K(lysine) acetyltransferase 2B, KAT2B, Lysine acetyltransferase 2B, P/CAF, P300/CBP associated factor, PCAF

---

Validation Data Gallery

• 

  WB analysis using 28770-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28770-1-AP (KAT2B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

---

Tested Applications

• Positive WB detected in: A431 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IP: See 1 publications below

Product Information

28770-1-AP targets PCAF in WB, IP, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PCAF fusion protein Ag30238
• Full Name: K(lysine) acetyltransferase 2B
• Calculated Molecular Weight: 93 kDa
• Observed Molecular Weight: 95-110 kDa
• GenBank Accession Number: BC060823
• Gene Symbol: PCAF/KAT2B
• Gene ID (NCBI): 8850
• RRID: AB_2918200
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IP	J Exp Clin Cancer Res; The histone lysine acetyltransferase KAT2B inhibits cholangiocarcinoma growth: evidence for interaction with SP1 to regulate NF2-YAP signaling; Authors - Wenbo Ma
human	WB	Cancer Cell; Tumor cells impair immunological synapse formation via central nervous system-enriched metabolite; Authors - Yihong Li
human	WB	J Cell Mol Med; SIRT2-mediated deacetylation of ACLY promotes the progression of oesophageal squamous cell carcinoma; Authors - Xueying Zhang