KAP1 Recombinant antibody, PBS Only (Detector)

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  Sandwich ELISA standard curve of MP02033-2

  Sandwich ELISA standard curve of MP02033-2, Human KAP1 Recombinant Matched Antibody Pair - PBS only. 85693-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag7350. 85693-2-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

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  Sample test of MP02033-2

  The mean KAP1 concentration was determined to be 262.9 ng/mL in HeLa cell extract based on a 1.5 mg/mL extract load, 240.8 ng/mL in HepG2 cell extract based on a 1.8 mg/mL extract load and 281.5 ng/mL in A549 cell extract based on a 3.1 mg/mL extract load.

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  Cytometric bead array standard curve of MP02033-1

  Cytometric bead array standard curve of MP02033-1, KAP1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85693-3-PBS. Detection antibody: 85693-2-PBS. Standard: Ag7350. Range: 0.625-80 ng/mL.

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  Cytometric bead array standard curve of MP02033-2

  Cytometric bead array standard curve of MP02033-2, KAP1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85693-1-PBS. Detection antibody: 85693-2-PBS. Standard: Ag7350. Range: 0.625-80 ng/mL.

KAP1 {ptg:Brand} Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 85693-2-PBS

Clone No.243043A7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

TRIM28, KRAB associated protein 1, KAP-1, KAP 1, EC:2.3.2.27

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Product Information

85693-2-PBS targets KAP1 as part of a matched antibody pair:

MP02033-1: 85693-3-PBS capture and 85693-2-PBS detection (validated in Cytometric bead array)

MP02033-2: 85693-1-PBS capture and 85693-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: KAP1 fusion protein Ag7350
• Full Name: tripartite motif-containing 28
• Calculated Molecular Weight: 89 kDa
• GenBank Accession Number: BC004978
• Gene Symbol: KAP1
• Gene ID (NCBI): 10155
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q13263
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.