KANSL3 Polyclonal antibody

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  WB analysis using 25533-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25533-1-AP (KANSL3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human intrahepatic cholangiocarcinoma using 25533-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 25533-1-AP (KANSL3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human intrahepatic cholangiocarcinoma using 25533-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 25533-1-AP (KANSL3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

KANSL3 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 25533-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

KIAA1310, KAT8 regulatory NSL complex subunit 3, PRTD, NSL3, NSL complex protein NSL3

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Tested Applications

• Positive WB detected in: HEK-293 cells, Jurkat cells, U2OS cells
• Positive IHC detected in: human intrahepatic cholangiocarcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25533-1-AP targets KANSL3 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KANSL3 fusion protein Ag22117
• Full Name: KIAA1310
• Calculated Molecular Weight: 878 aa, 93 kDa
• Observed Molecular Weight: 105 kDa
• GenBank Accession Number: BC063792
• Gene Symbol: KANSL3
• Gene ID (NCBI): 55683
• RRID: AB_3085802
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9P2N6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KANSL3, or KAT8-associated nonspecific lethal complex subunit 3, is a component of the KANSL complex, which is an important chromatin modifier in higher animals. This complex plays a crucial role in regulating housekeeping genes and is highly conserved across species. KANSL3, along with other KANSL complex members, is essential for cell division and has been shown to interact robustly both in vitro and in vivo. KANSL3 has been found to have a dual role in cellular function. In interphase, KANSL3 is chromatin-bound and regulates the expression of housekeeping genes. During mitosis, KANSL3 relocates to the mitotic spindle and is important for cell division. It has been identified as a microtubule minus-end-binding protein, which is a novel function for a chromatin-binding complex. KANSL3's ability to bind directly to microtubule minus ends is significant, as very few proteins are known to perform this function.